The invention discloses a kit for endometrial cancer detection for an endometrial tissue sample. The detection is based on the activation mutation of a specific site in a PTEN gene. The invention relates to an endometrial cancer detection kit and probe for endometrial cancer detection based on related nucleotide sequences of the PTEN mutation site.

Endometrial cancer gene detection kit

The present invention provides a non-invasive detection kit for detecting endometrial cancer predisposing gene. The kit comprises specific primers for detecting genotypes of three single nucleotide polymorphism sites, DNA sequencing primers, a PCR reaction component, a PCR product purification component, a DNA sequencing reaction component, and the like, wherein the three single nucleotide polymorphism sites comprise MspI of CYP1A1 gene, Leu432Val of CYP1B1 gene, and MspA1I of CYP17gene. According to the present invention, the kit of the present invention detects the genotypes of the three single nucleotide polymorphism sites closely related to the development of the endometrial carcinoma so as to evaluate the risk level of the development of the endometrial carcinoma of the subject, and guide the female to targeted prevent the development of the endometrial carcinoma. The sampling method of the present invention is the oral mucosa cell sampling, such that characteristics of painlessness and no invasion are provided, and the cross infection is avoided. With the present invention, the sequencing result is accurate and reliable, the expensive imported equipment is not required to purchase, and the method is easy to popularize.

Non-invasive detection kit for endometrial carcinoma predisposing gene

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Non-invasive detection kit for endometrial carcinoma predisposing gene