In the present study, we report graphene quantum dots (GQDs), an enzyme mimetic of horse radish peroxidase (HRP), for unprecedented detection of free cholesterol. Synthesized directly from graphite using simple and quick one step wet chemical method, these GQDs in the presence of H 2 O 2 exhibit highly efficient catalytic activity toward the oxidation of peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) to produce a blue colored product. The proposed detection system based on GQDs allows wide range (0.02–0.6 mM) of cholesterol sensing with a detection limit as low as 0.006 mM. Further, higher V max (7.3 × 10 −6  M s −1 ) along with lower K m (0.01 mM) attest enhanced peroxidase like catalytic activity and better binding affinity of cholesterol oxidase (ChOx) to cholesterol resulting in good biosensor stability and resistance to environmental interferences. The proposed method without the use of sophisticated instruments perceives the cholesterol using naked eye with blue color compound formation. The potential of the method to be applied on field is shown by the proposed cholesterol measuring color wheel, where the shades of color are related to actual levels of cholesterol in the sample.

Colorimetric detection of cholesterol based on highly efficient peroxidase mimetic activity of graphene quantum dots

Cholesterol biosensors: A review.

Amperometric cholesterol biosensor based on the direct electrochemistry of cholesterol oxidase and catalase on a graphene/ionic liquid-modified glassy carbon electrode

The novel boron nitride nanosheet and copper sulfide nanohybrids (BNNS@CuS) were synthesized by a facile solvothermal approach and proposed as a prominent peroxidase-like mimic. Through integrating an intensified colour reaction induced by catalytical oxidation with cholesterol oxidase, the BNNS@CuS was developed for the colorimetric determination of cholesterol with a linear range of 10–100 μM and a detection limit of 2.9 μM. The intrinsic peroxidase-like activity of BNNS@CuS followed the typical Michaelis–Menten kinetics. The method was further utilized for the visual detection of total cholesterol in human serum and showed high selectivity toward cholesterol. Our research manifested that the cholesterol biosensing method based on BNNS@CuS was cost-effective, reliable, highly efficient and feasible.

Boron nitride nanosheet/CuS nanocomposites as mimetic peroxidase for sensitive colorimetric detection of cholesterol

MXene-Ti3C2/CuS nanocomposites: Enhanced peroxidase-like activity and sensitive colorimetric cholesterol detection.

Highly sensitive and selective cholesterol biosensor based on direct electron transfer of hemoglobin

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Highly sensitive and selective cholesterol biosensor based on direct electron transfer of hemoglobin