7 Papers
4 Citations
Li A is an academic researcher from Stanford University. The author has contributed to research in topics: Aptamer & Patched. The author has an hindex of 3, co-authored 7 publications.
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Papers
Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit.
Yunxiao Zhang,Wan Jin Lu,David Bulkley,Jiahao Liang,Arthur Ralko,Shuo Han,Kelsey J. Roberts,Li A,Wonhwa Cho,Yifan Cheng,Yifan Cheng,Aashish Manglik,Philip A. Beachy +12 more
TL;DR: An agonist is developed, a conformation-specific nanobody that potentially targets the conformational changes of the Hedgehog receptor Patched1 and potently activates the pathway in vivo, facilitating mechanistic studies of Hedgehog pathway activation and the engineering of pathway activating agents for therapeutic use.
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Identification and mitigation of pervasive off-target activity in CRISPR-Cas9 screens for essential non-coding elements
Josh Tycko,Michael Wainberg,Georgi K. Marinov,Oana Ursu,Gaelen T. Hess,Braeden K. Ego,Aradhana,Li A,Truong A,Alexandro E. Trevino,Kaitlyn Spees,David Yao,Irene M. Kaplow,Peyton Greenside,David W. Morgens,Douglas H. Phanstiel,Douglas H. Phanstiel,Snyder M,Lacramioara Bintu,William J. Greenleaf,Anshul Kundaje,Michael C. Bassik +21 more
TL;DR: Results show off-target activity can severely limit identification of essential functional motifs by active Cas9, while strictly filtered CRISPRi screens can be reliably used for assaying larger regulatory elements.
Hedgehog pathway activation through conformational blockade of the Patched sterol conduit
Yunxiao Zhang,Wan Jin Lu,David P. Bulkley,Jiahao Liang,Arthur Ralko,Kelsey J. Roberts,Li A,Wonhwa Cho,Yifan Cheng,Yifan Cheng,Aashish Manglik,Philip A. Beachy +11 more
TL;DR: The conformation-selective nanobody approach provides a new route to the development of transporter-related pharmacologic agents and may be generally applicable to the study of other transporters.
Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms
Alex M. Yoshikawa,Alexandra E. Rangel,Trevor A. Feagin,Chun E,Leighton Wan,Li A,Moekl L,Michael Eisenstein,Sharon J. Pitteri,Hyongsok Tom Soh +9 more
TL;DR: In this article, a fluorescence-activated cell sorting (FACS)-based approach was used to generate and screen aptamers with indole-modified bases in an effort to isolate reagents that can differentiate between protein glycoforms.
3
Systematic identification of regulators of antibody-drug conjugate toxicity using CRISPR-Cas9 screens
C. K. Tsui,Robyn M. Barfield,Curt R. Fischer,David W. Morgens,Li A,Smith Bah,Carolyn R. Bertozzi,Carolyn R. Bertozzi,David Rabuka,Michael C. Bassik +9 more
TL;DR: It is shown that a subset of late endosomal and lysosomal regulators are selectively essential for toxicity of noncleavable linker ADCs, and that inhibition of sialic acid biosynthesis sensitizes cells to ADC treatment by increasing the rate of ADC internalization.