30 Papers
47 Citations
Lei Han is an academic researcher from Shanghai Jiao Tong University. The author has contributed to research in topics: Antibody & Intein. The author has an hindex of 9, co-authored 23 publications.
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Papers
RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis.
Chaodong Li,Mei Ge,Daijie Chen,Tao Sun,Hua Jiang,Yueqing Xie,Huili Lu,Baohong Zhang,Lei Han,Junsheng Chen,Jianwei Zhu +10 more
TL;DR: It is suggested that siRNA against the RPL21 gene possesses a potential anti-cancer activity for PC cells by inhibiting their proliferation and DNA replication, as well as inducing cell cycle G1 arrest and cell apoptosis.
Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein
TL;DR: It has been demonstrated that different TGE batches are reproducible from lot to lot in overall cell growth, product yield, and product qualities including isoelectric point, binding affinity, secondary structure, and thermal stability.
30
Producing defucosylated antibodies with enhanced in vitro antibody-dependent cellular cytotoxicity via FUT8 knockout CHO-S cells
Huifang Zong,Lei Han,Ding Kai,Jiaxian Wang,Tao Sun,Xinyu Zhang,Cedric Cagliero,Hua Jiang,Yueqing Xie,Jianrong Xu,Baohong Zhang,Jianwei Zhu +11 more
TL;DR: The results demonstrated the potential of using the clustered regularly interspaced short palindromic repeats‐CRISPR associated nuclease 9 technology in cell line engineering for biopharmaceutical industrial applications.
22
Enhanced production of anti-PD1 antibody in CHO cells through transient co-transfection with anti-apoptotic genes Bcl-x L and Mcl-1
Xinyu Zhang,Lei Han,Huifang Zong,Ding Kai,Yuan Yuan,Jingyi Bai,Yuexian Zhou,Baohong Zhang,Jianwei Zhu +8 more
TL;DR: This work provides an effective way to increase viability of host cells through delaying apoptosis onset, thus, raise production yield of biopharmaceuticals without the process of generating stable cell lines and subsequent screening.
22
The chemokine CXCL9 exacerbates chemotherapy-induced acute intestinal damage through inhibition of mucosal restitution
Huili Lu,Hongyu Liu,Jiaxian Wang,Jiaqing Shen,Shunyan Weng,Lei Han,Tao Sun,Lan Qian,Mingyuan Wu,Shunying Zhu,Yan Yu,Wei Han,Jianwei Zhu,Anja Moldenhauer +13 more
TL;DR: CXCL9 inhibits the proliferation of epithelial cells via phosphorylation of p70S6K, resulting in the excretion of TGF-β as downstream mediator, and anti-CxCL9 agents are potential novel therapeutic candidates for promoting mucosal restitution.
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