Laura A. Banaszynski
University of Texas Southwestern Medical Center
47 Papers
282 Citations
Laura A. Banaszynski is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: Chromatin & Biology. The author has an hindex of 20, co-authored 37 publications. Previous affiliations of Laura A. Banaszynski include Stanford University & Rockefeller University.
Chat about Author
Papers
Genome editing a mouse locus encoding a variant histone, H3.3B, to report on its expression in live animals.
Duancheng Wen,Kyung-Min Noh,Aaron D Goldberg,C. David Allis,Zev Rosenwaks,Shahin Rafii,Laura A. Banaszynski +6 more
TL;DR: ZFN mediated gene editing is used to introduce a small C‐terminal hemagglutinin (HA) tag to the endogenous H3.3B locus in mouse embryonic stem cells, along with an internal ribosome entry site (IRES) and a separately translated fluorescent reporter of expression that will allow detection of expression driven by the reporter in cells, animals, and embryos.
Recent Progress with FKBP-Derived Destabilizing Domains
TL;DR: The FKBP-derived destabilizing domains are increasingly being used to confer small molecule-dependent stability to many different proteins, however these engineered destabilization domains are not dominant to endogenous degrons that regulate protein stability.
PASK links cellular energy metabolism with a mitotic self-renewal network to establish differentiation competence
Michael Xiao,Chia Hua Wu,Graham Meek,Brian Kelly,Dara Buendia Castillo,Lyndsay E.A. Young,Sara Martire,Sajina Dhungel,Purbita Saha,Altair L. Dube,Matthew S. Gentry,Laura A. Banaszynski,Ramon C. Sun,Chintan K. Kikani +13 more
TL;DR: In this article , a pathway downstream of mitochondrial glutamine metabolism that confers stem cell heterogeneity and establishes differentiation competence by countering post-mitotic self-renewal machinery is described.
A general method for conditional regulation of protein stability in living animals.
Mark A. Sellmyer,Steve H. Thorne,Steve H. Thorne,Laura A. Banaszynski,Christopher H. Contag,Thomas J. Wandless +5 more
TL;DR: This protocol uses tumor xenograftsin mice as one such mechanism for delivering the fusion protein and presents a method for delivering Shield-1 to regulate the fusion proteins in vivo.
Differential contribution of p300 and CBP to regulatory element acetylation in mESCs.
Sara Martire,Jennifer Nguyen,Aishwarya Sundaresan,Laura A. Banaszynski +3 more
- 20 Jul 2020
TL;DR: It is demonstrated that p300 is the predominant H3K27 acetyltransferase in mESC and that loss of acetylation in p300KD mESCs is more pronounced at enhancers compared to promoters.