L. Sobotková
Academy of Sciences of the Czech Republic
5 Papers
27 Citations
L. Sobotková is an academic researcher from Academy of Sciences of the Czech Republic. The author has contributed to research in topics: Escherichia coli & Plasmid. The author has an hindex of 3, co-authored 4 publications.
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Papers
Development of a high-expression system for penicillin G acylase based on the recombinant Escherichia coli strain RE3(pKA18)
TL;DR: The high-expression system developed using the high PGA-producing strain Escherichia coli RE3 as a host and a recombinant plasmid pKA18 which was constructed by cloning the chromosomal pga gene coding for PGA in the strain RE3 on multicopy vector pK19 led to an increase in the specific activity of PGA.
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Cloning of penicillin G acylase-encoding gene from Escherichia coli high-producing strain RE3
TL;DR: The highest constitutive expression of pga was observed in a strain bearing the recombinant plasmid pKA18 synthesizing 2.5 times more of enzyme than the parent strain.
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Global Impact of the COVID-19 Pandemic on Stroke Volumes and Cerebrovascular Events
Thanh N. Nguyen,Muhammad M. Qureshi,Piers Klein,Hiroshi Yamagami,Robert Mikulik,Anna Członkowska,Mohamad Abdalkader,Petra Sedova,Anvitha Sathya,Hannah C Lo,Ossama Mansour,Husitha Vanguru,E. Lesaine,Georgios Tsivgoulis,Aaron Loochtan,Jelle Demeestere,Ken Uchino,Violiza Inoa,Nitin Goyal,Andreas Charidimou,James E. Siegler,Shadi Yaghi,Diana Aguiar de Sousa,Mahmoud H Mohammaden,Diogo C Haussen,Espen Saxhaug Kristoffersen,Virginia Pujol Lereis,Sergio Scollo,Bruce C.V. Campbell,Alice Ma,J. Thomas,Mark W Parsons,Shaloo Singhal,Lee-Anne Slater,Rodrigo Tomazini Martins,Christian Enzinger,Thomas Gattringer,Amir M. Rahmani,Thomas Bonnet,Noémie Ligot,Sylvie De Raedt,Robin Lemmens,Peter Vanacker,Fenne Vandervorst,Adriana Bastos Conforto,Raquel Hidalgo,Luciana de Oliveira Neves,Rodrigo Targa Martins,Daissy Liliana Mora Cuervo,Leticia C Rebello,I. Santiago,Isabelle Lameirinhas da Silva,T. Sakelarova,Rosen Kalpachki,Filip Alexiev,Luciana Catanese,Elena A. Cora,Mayank Goyal,Michael D. Hill,Michael Kelly,Houman Khosravani,Pascale Lavoie,Lissa Peeling,Aleksandra Pikula,Rodrigo Arturo Marquet Rivera,Hui-sheng Chen,Yimin Chen,Xiaochuan Huo,Zhongrong Miao,Shuiquan Yang,Marina Roje Bedeković,Marina Bralic,Hrvoje Budinčević,Angel Basilio Corredor-Quintero,O. Lara-Sarabia,Martin Čábal,D. Tenora,Petr Fibrich,Roman Herzig,Helena Hlaváčová,Emanuela Hrabanovska,David Hlinovský,Lubomír Jurák,Jana Kadlcikova,I. Karpowicz,Lukas Klecka,Martin Kovar,D. Lauer,Jiří Neumann,Hana Palouskova,M. F. Reiser,Petra Reková,Vladimir Rohan,Ondřej Škoda,Miroslav Skorna,L. Sobotková,Martin Šrámek,Lenka Žáková,Hanne Christensen,Nicolas Drenck,H. Iversen,Thomas Truelsen,Troels Wienecke,Khalid M Sobh,Pauli Ylikotila,Kemal Alpay,Daniel Strbian,Patricia Bernady,P Casenave,Maria Bostenaru Dan,Jean-Marc Faucheux,Jean-Christophe Gentric,Elsa Magro,Candice Sabben,Peggy Reiner,François Rouanet,Ferdinand O Bohmann,Stefan Boskamp,Joshua Mbroh,Simon Nagel,Christian H. Nolte,Peter A. Ringleb,Michael Rosenkranz,Sven Poli,Götz Thomalla,Theodoros Karapanayiotides,Ioanna Koutroulou,Odysseas Kargiotis,Lina Palaiodimou,Jose Dominguo Barrientos Guerra,Vikram Huded,Bindu Menon,Shashank Nagendra,Chintan Prajapati,P N Sylaja,Nyoman Angga Krishna Pramana,Achmad Firdaus Sani,Abdoreza Ghoreishi,Mehdi Farhoudi,Elyar Sadeghi Hokmabadi,Tariq Abu Raya,Shani Avnery Kalmanovich,Levite Ronen,Sergiu Sabetay,Maurizio Acampa,Alessandro Adami,Lucio Castellan,Marco Longoni,Raffaele Ornello,Leonardo Renieri,C. Rolla Bigliani,Michele Romoli,Simona Sacco,Andrea Salmaggi,Davide Sangalli,Andrea Zini,Ryosuke Doijiri,Hiroki Fukuda,Toshiyuki Fujinaka,Kyohei Fujita,Hirotoshi Imamura,Nobuyuki Sakai,Takuya Kanamaru,Naoto Kimura,Ryuhei Kono,Kosuke Miyake,Manabu Sakaguchi,Kenichiro Sakai,Kazutaka Sonoda,Kenichi Todo,Fumio Miyashita,Naoki Tokuda,Yuji Matsumaru,Shoji Matsumoto,Nobuyuki Ohara,Seigo Shindo,Yohei Takenobu,Takashi Yoshimoto,Kazunori Toyoda,Takeshi Uwatoko,Yoshiki Yagita,Takehiro Yamada,Nobuaki Yamamoto,Ryoo Yamamoto,Yukako Yazawa,Yuri Sugiura,Peter Kuria Waweru,Jang Hyun Baek,Si Baek Lee,Kwon-Duk Seo,Sung Il Sohn,Anita Arsovska,Yong Chieh Chan,Wan Asyraf Wan Zaidi,Ainul Syahrilfazli Jaafar,Fernando Góngora-Rivera,Manuel Martínez-Marino,Adrian Infante-Valenzuela,Stanislav Groppa,P Leahu,Jonathan M. Coutinho,Leon A. Rinkel,Diederik W.J. Dippel,Dianne H.K. van Dam-Nolen,Annemarei Ranta,Teddy Y. Wu,Tajudeen Temitayo Adebayo,Abiodun H Bello,Ernest O. Nwazor,Taofiki Sunmonu,Kolawole Wahab,Ole Morten Rønning,Else Charlotte Sandset,Amal M Al Hashimi,Saima Ahmad,Umair Rashid,Liliana Rodríguez-Kadota,M.A. Vences,P. M. Yalung,Jon Stewart Hao Dy,Maria Carissa C. Pineda-Franks,Christian Co,Waldemar Brola,Aleksander Dębiec,Małgorzata Dorobek,Michał Karliński,Beata Labuz-Roszak,Anetta Lasek-Bal,Halina Sienkiewicz-Jarosz,Jacek Staszewski,Piotr Sobolewski,Marcin Wiącek,Justyna Zielińska-Turek,André Araújo,Mariana Rocha,Pedro M. Castro,Vítor Tedim Cruz,Paulo Venancio Ferreira,Patrícia Ferreira,Ana Paiva Nunes,Luísa Fonseca,João Marto,Teresa M. V. D. Pinho e Melo,Miguel Angelo Rodrigues,M. Silva,Adela Dimitriade,Cristian Falup-Pecurariu,May Adel Hamid,Narayanaswamy Venketasubramanian,Georgi Krastev,Miroslav Mako,Óscar Ayo-Martín,Francisco Hernández-Fernández,Jordi Blasco,A. Rodríguez-Vázquez,A. Cruz-Culebras,Francisco Moniche,Joan Antoni Martín Montaner,Soledad Pérez-Sánchez,M. G. García Sánchez,M. Rodríguez,K. Joo,Annika Nordanstig,Michael V. Mazya,Tiago Moreira,Gianmarco Bernava,Morin Beyeler,Manuel Bolognese,Emmanuel Carrera,Tomas Dobrocky,G M Karwacki,Emanuela Keller,Chang Yang Hsieh,Surawan Boonyakarnkul,Anchalee Churojana,O. Aykaç,A. Ozcan Ozdemir,Arsida Bajrami,Songul Senadim,Syed Irteza Hussain,Seby John,Soma Banerjee,Joseph Kwan,K. Ranga Rama Krishnan,R. Lenthall,Ashok Matthews,Ken Wong,Liqun Zhang,Dorothea Altschul,Kaiz Asif,Zeelalem Bahiru,Kristine Below,José Biller,Sean Ruland,Saqib Chaudry,Michael Chen,Alex Bou Chebl,Jackie Cibulka,Leon Cistrunk,Judith Clark,Marco Colasurdo,Alexandra L Czap,Adam de Havenon,Salvatore A. D’Amato,S Dharmadhikari,Kasey B Grimmett,Adam A Dmytriw,Mark R Etherton,Chizoba Ezepue,Mudassir Farooqui,Steven K. Feske,Lauren Fink,Ulviyya Gasimova,Amy K Guzik,Maryam Hakemi,Majesta Hovingh,Muhib Khan,Dinesh Jillela,Peter Kan,Rakesh Khatri,Ayaz Khawaja,Naim Khoury,Nicole L Kiley,Benny S. Kim,Murali K Kolikonda,A. Kuhn,Stephanie V. Lara,Guillermo Linares,Italo Linfante,Timothy G. Lukovits,Sarah Lycan,Shailesh Male,Laith Maali,John Mancin,Hesham Masoud,Ghada A. Mohamed,Andre Monteiro,Fadi Nahab,Krishna Nalleballe,Santiago Ortega Gutierrez,Ajit S. Puri,Yazan Radaideh,Rahul Rahangdale,Ansaar T Rai,Pankajavalli Ramakrishnan,A Bhaskara Reddy,Diana Rojas-Soto,Jose R. Romero,Natalia S. Rost,Aaron Rothstein,Setareh Salehi Omran,Sunil A Sheth,Adnan H. Siddiqui,Amy K Starosciak,Nicholas Tarlov,Robert W. Taylor,Michael J Wang,Jared Wolfe,Ka-Ho Wong,H.-V. Le,Quy Viet Nguyen,Thong Pham,Trung Thanh Nguyen,Hoang T Phan,Mai Duy Ton,H. Fischer,Patrik Michel,Davide Strambo,Sheila Cristina Ouriques Martins,Osama O. Zaidat,Raul G Nogueira +370 more
TL;DR: The results suggest preservation in the stroke care of higher severity of disease through the first pandemic year and a global decline and shift to lower volume centers of stroke admission volumes, ICH volumes, and IVT volumes during the 1st year of the COVID-19 pandemic compared to the prior year.
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Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W.
TL;DR: Three indigenous plasmids identified among producers of penicillin G acylase, (PGA) derived from the strain Escherichia coli W ATCC 9637 did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
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Evaluation of strains derived from Escherichia coli W as hosts for the expression of penicillin G-acylase-encoding gene cloned on the recombinant plasmid pKA18.
TL;DR: The host strain RE3 showing the highest production of PGA was also the best host of the recombinant plasmid in terms of the segregational stability and copy number (198 copies per chromosome).
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