Kunihiko Yoshimura
National Institutes of Health
11 Papers
365 Citations
Kunihiko Yoshimura is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Cystic fibrosis transmembrane conductance regulator & Genetic enhancement. The author has an hindex of 9, co-authored 11 publications.
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Papers
Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis
Bruce C. Trapnell,Chin-Shyan Chu,Paavo K. Paakko,Tyrone C. Banks,Kunihiko Yoshimura,Victor J. Ferrans,Milica S. Chernick,Ronald G. Crystal +7 more
TL;DR: Estimation of CFTR gene expression quantified by limited polymerase chain reaction amplification showed that in normal individuals, CFTR mRNA transcripts are expressed in nasal, tracheal, and bronchial epithelial cells at approximately 1-2 copies per cell, more than 100-fold greater than in pharyngeal epithelium.
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Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer
Kunihiko Yoshimura,Melissa A. Rosenfeld,Hidenori Nakamura,Eva M. Scherer,Andrea Pavirani,Jean-Pierre Lecocq,Ronald G. Crystal +6 more
TL;DR: In vivo evaluation of beta-galactosidase activity after intratracheal administration of an E. coli lacZ gene expression plasmid vector directed by the cytomegalovirus promoter demonstrated that the airway epithelium was the major target of transfer and expression of the exogenous gene.
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Patent
Method of adenoviral-medicated cell transfection
Prem Seth,Ronald G. Crystal,Melissa A. Rosenfeld,Kunihiko Yoshimura +3 more
- 04 Feb 1994
TL;DR: In this paper, an adenoviral-mediated method of transfection with nucleic acids which can be augmented through incubation of the nucleic acid with cationic agents is presented.
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Gene transfer to freshly isolated human respiratory epithelial cells in vitro using a replication-deficient adenovirus containing the human cystic fibrosis transmembrane conductance regulator cDNA.
Melissa A. Rosenfeld,Chin-Shyan Chu,Prem Seth,Claire Danel,Tyrone C. Banks,Koichi Yoneyama,Kunihiko Yoshimura,Ronald G. Crystal +7 more
TL;DR: The present study demonstrates that AdCFTR restores cAMP-stimulated Cl- permeability in human CF bronchial epithelial cells and suggests that biologic efficacy should be achievable in vivo.
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Down-regulation of cystic fibrosis transmembrane conductance regulator gene expression by agents that modulate intracellular divalent cations.
Joachim Bargon,Bruce C. Trapnell,Chin Shyan Chu,Eugene Rosenthal,Kunihiko Yoshimura,William B. Guggino,Wilfried Dalemans,Andrea Pavirani,J P Lecocq,Ronald G. Crystal +9 more
TL;DR: Increases in intracellular divalent cation concentrations down-regulate the expression of the CFTR gene at the transcriptional level, with consequent decreases in CFTR mRNA and protein.
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