Although the protein synthesis inhibitor cycloheximide (CHX) has been known for decades, its precise mechanism of action remains incompletely understood. The glutarimide portion of CHX is seen in a family of structurally related natural products including migrastatin, isomigrastatin and lactimidomycin (LTM). We found that LTM, isomigrastatin and analogs have a potent antiproliferative effect on tumor cell lines and selectively inhibit translation. A systematic comparative study of the effects of CHX and LTM on protein synthesis revealed both similarities and differences between the two inhibitors. Both LTM and CHX were found to block the translocation step in elongation. Footprinting experiments revealed protection of a single cytidine nucleotide (C3993) in the E-site of the 60S ribosomal subunit, thus defining a common binding pocket for the two inhibitors in the ribosome. These results shed new light on the molecular mechanism of inhibition of translation elongation by both CHX and LTM.

/pdf/inhibition-of-eukaryotic-translation-elongation-by-6ozzttmjnv.pdf

Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin

Biosynthesis of polyketides by trans-AT polyketide synthases

A systematic analysis of all naturally-occurring glycosylated bacterial secondary metabolites reported in the scientific literature up through early 2013 is presented. This comprehensive analysis of 15 940 bacterial natural products revealed 3426 glycosides containing 344 distinct appended carbohydrates and highlights a range of unique opportunities for future biosynthetic study and glycodiversification efforts.

/pdf/a-comprehensive-review-of-glycosylated-bacterial-natural-4txxvyk4h3.pdf

A comprehensive review of glycosylated bacterial natural products.

Polyphasic taxonomy of the genus Talaromyces

Velocity Fields in a Collectively Migrating Epithelium

Wehave isolated migrastatin (Fig. 1), as an inhibitor of tumor cell migration, from a cultured broth of Streptomyces sp. MK929-43F1. In the preceding paper1}, the taxonomy, fermentation, isolation and biological activities were reported. In this paper, we describe the physico-chemical properties and structure elucidation of migrastatin. Physico-chemical properties of migrastatin are summarized in Table 1. Migrastatin is readily soluble in methanol, acetone, ethyl acetate, chloroform and DMSO and practically insoluble in water and?z-hexane. Migrastatin was isolated as a white powderwith melting point of 54~55°C. The UV spectrum showed end absorption. Migrastatin gave positive color reaction with molybdophosphoric acid-sulfuric acid and 2,4-dinitrophenylhydrazine but negative with ninhydrin and Rydon-Smith. The molecular formula for migrastatin was established as C27H39NO7 by HRFAB-MS, which was supported by the *H and 13C NMRspectra. The DEPT and HMQCspectra exhibited 27 resonances; three methyl, one methoxy, seven methylene, five methine, one oxygen bearing methine, five olefinic methine, one quaternary olefinic and four carbonyl carbons. The !H NMRspectrum exhibited the presence of two deuterium exchangeable protons other than the protons which were attributed to the carbons described above. These !H and 13C chemical shifts are listed in Table 2.

Migrastatin, a novel 14-membered lactone from Streptomyces sp. MK929-43F1.

migrastatin isolate contained teleocidin-related compounds which display strong inhibitory activity against tumor cell migration. In this report, we describe the effects of teleocidin-free migrastatin on tumor cell migration and on the growth of several types of tumor cells. Whenthe original migrastatin sample was subjected to HPLC (Capcell Pak C18 column, 20X250mm) developing with 70% aq acetonitrile, a potent migration inhibitory activity was eluted in a fraction other than migrastatin, indicating that the migration inhibitory activity of our

Migrastatin, a novel 14-membered ring macrolide, inhibits anchorage-independent growth of human small cell lung carcinoma Ms-1 cells.

Absolute configuration of migrastatin, a novel 14-membered ring macrolide.

https://www.cell.com/cell-chemical-biology/pdf/S1074-5521(05)00346-7.pdf

Chemistry and Biology of Moverastins, Inhibitors of Cancer Cell Migration, Produced by Aspergillus

Migrastatin and its analogs have various biological activities such as inhibition of cell migration and anchorage-independent growth of cancer cells Although its biosynthesis and chemical synthesis have been under investigation, little is known about the biological target of migrastatin Here, we found that migrastatin inhibited intracellular calcium mobilization induced by carbachol in neuroblastoma SK-N-SH cells without affecting Ca2+ mobilization and cAMP accumulation induced by ligands of other receptors The binding of [3H] N-methyl- scopolamine, an antagonist for muscarinic receptor was also inhibited by migrastain Functionally, migrastatin inhibited Ca2+ mobilization induced by carbachol in primary cultures of smooth muscle cells of rat bladder This study reveals that migrastatin acts as a muscarinic acetylcholine receptor antagonist

/pdf/migrastatin-acts-as-a-muscarinic-acetylcholine-receptor-5bbkw2k9om.pdf

Migrastatin Acts as a Muscarinic Acetylcholine Receptor Antagonist

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Koichi Nakae

Author Tools

Chat about Author