Karla Ilić Đurđić
University of Belgrade
9 Papers
7 Citations
Karla Ilić Đurđić is an academic researcher from University of Belgrade. The author has contributed to research in topics: Saturated mutagenesis & Peroxidase. The author has an hindex of 4, co-authored 9 publications.
Chat about Author
Papers
Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization
Nikolina Popović,Dunja Pržulj,Maja Mladenovic,Olivera Prodanović,Selin Ece,Karla Ilić Đurđić,Raluca Ostafe,Rainer Fischer,Radivoje Prodanovic +8 more
TL;DR: Cell wall laccase immobilized within dopamine-alginate beads decolorized Amido Black 10B, Reactive Black 5, Evans Blue, and Remazol Brilliant Blue with 100% efficiency and after ten rounds of multiple-use retained decolorization efficiency of 90% with Evans Blue and 61% with AmidoBlack.
37
Saturation mutagenesis to improve the degradation of azo dyes by versatile peroxidase and application in form of VP-coated yeast cell walls
Karla Ilić Đurđić,Raluca Ostafe,Aleksandra Đurđević Đelmaš,Nikolina Popović,Stefan Schillberg,Rainer Fischer,Radivoje Prodanovic +6 more
TL;DR: Saturation mutagenesis was used to alter two amino acids in the catalytic tryptophan environment of VP to isolate and sequence VP variants with up to 16-fold higher catalytic efficiency for different azo dyes, making this platform ideal for the bioremediation of environments contaminated with azoDyes.
34
A High-Throughput Screening System Based on Droplet Microfluidics for Glucose Oxidase Gene Libraries.
Radivoje Prodanovic,Radivoje Prodanovic,W. Lloyd Ung,Karla Ilić Đurđić,Rainer Fischer,David A. Weitz,Raluca Ostafe +6 more
TL;DR: A new ultrahigh-throughput screening platform based on a microfluidic lab-on-chip device that allows the sorting of GOx mutants from a saturation mutagenesis library expressed on the surface of yeast cells that was more sensitive than flow cytometry and supports comprehensive screens of gene libraries containing multiple mutations per gene.
26
Optimization of phenol removal with horseradish peroxidase encapsulated within tyramine-alginate micro-beads
Nevena Pantić,Radivoje Prodanovic,Karla Ilić Đurđić,Natalija Polovic,Milica Spasojević,Olivera Prodanović +5 more
TL;DR: An internal generation of hydrogen peroxide from glucose and glucose oxidase was employed in the phenol removal process with HRP immobilized on tyramine-alginate for the first time, achieving a high removal efficiency and substantial reusability.
24
Improvement in oxidative stability of versatile peroxidase by flow cytometry-based high-throughput screening system
Karla Ilić Đurđić,Selin Ece,Selin Ece,Raluca Ostafe,Raluca Ostafe,Simon Vogel,Stefan Schillberg,Rainer Fischer,Rainer Fischer,Radivoje Prodanovic +9 more
TL;DR: It is confirmed that the high-throughput screening system can improve the oxidative stability of versatile peroxidase, providing a source of novel enzymes for remediation applications.
12