Karin Heurlier

TL;DR: This work has designed a series of donor plasmids for use with the λ-Red recombination system, which when cleaved in vivo by the I-SceI meganuclease generate a discrete linear DNA fragment, allowing for C-terminal tagging of chromosomal genes with a 6 × His, 3 × FLAG, 4 × ProteinA or GFP tag or for the deletion of chromosome regions.

TL;DR: In vitro, lasR mutants have a higher probability to escape lytic death in stationary phase under alkaline conditions than has the QS-proficient wild type, raising the question of whether the quorum-sensing machinery can have a negative impact on the organism's fitness.

TL;DR: In this paper, it was shown that the Pfs reaction is the sole intracellular source of the AI-2 signal in Neisseria meningitidis mutants, and that the observed growth defect is not due to quorum sensing but is probably due to metabolic imbalance and, in the case of pfs activation, is most likely due to toxic accumulation of SAH.

TL;DR: It is shown that the Pfs reaction is the sole intracellular source of the AI-2 signal and the observed growth defect of N. meningitidis pfs and luxS mutants is not due to quorum sensing but is probably due to metabolic imbalance and, in the case of pfs inactivation, is most likely due to toxic accumulation of SAH.