Karin Flükiger
University of Bern
8 Papers
187 Citations
Karin Flükiger is an academic researcher from University of Bern. The author has contributed to research in topics: PEP group translocation & Protein subunit. The author has an hindex of 8, co-authored 8 publications.
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Papers
Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS)
TL;DR: The IICBGlc subunit spans the membrane eight times as discussed by the authors and was 30 times more active when two proteins were encoded by a dicistronic mRNA than by two genes.
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Functional Reconstitution of the Purified Mannose Phosphotransferase System of Escherichia coli into Phospholipid Vesicles
TL;DR: A new plasmid pTSHIC9 for the controlled overexpression of the cytoplasmic phosphoryl carrier proteins, enzyme I, HPr, and IIAGlc, and a simplified procedure for the purification of these proteins are also described.
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Mechanism of Phosphoryl Transfer in the Dimeric IIABMan Subunit of the Escherichia coliMannose Transporter
TL;DR: Both cis andtrans pathways contribute to the maximal phosphotransferase activity of IIABMan, which occurs as a stable homodimer and heterodimers of different subunits were produced in vitro by GuHCl-induced unfolding and refolding of mixtures of two different homodimer.
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Mechanism-based Inhibition of Enzyme I of the Escherichia coli Phosphotransferase System CYSTEINE 502 IS AN ESSENTIAL RESIDUE
Luis F. Garcia-Alles,Karin Flükiger,Johannes Hewel,Regula Gutknecht,Christian Siebold,Stefan Schürch,Bernhard Erni +6 more
TL;DR: Four phosphoenolpyruvate derivatives, carrying reactive or activable chemical functions in each of the three chemical regions of PEP, were assayed as alternative substrates of enzyme I (EI) of the Escherichia coliPEP:glucose phosphotransferase system, highlighting the importance of these functionalities for recognition by EI.
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Purification and Electron Microscopic Characterization of the Membrane Subunit (IICBGlc) of the Escherichia coli Glucose Transporter
TL;DR: Single particle analysis of IICB(Glc) in proteoliposomes obtained by detergent dialysis also showed predominantly dimeric structures.
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