Kaiyi Jiang
9 Papers
Kaiyi Jiang is an academic researcher. The author has contributed to research in topics: Biology & RNA. The author has an hindex of 4, co-authored 6 publications.
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Papers
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Matthew T. N. Yarnall,Eleonora I. Ioannidi,Cian Schmitt-Ulms,Rohan N. Krajeski,Justin K. Lim,Lukas Villiger,Wenyuan Zhou,Kaiyi Jiang,Sofya K. Garushyants,Nathan Roberts,Liyang Zhang,Christopher A. Vakulskas,John A. Walker,Anastasia P. Kadina,Adrianna E Zepeda,Kevin Holden,Hong-Yu Ma,Jun Xie,Guangping Gao,Lander Foquet,Greg Bial,Sara K. Donnelly,Yoshinari Miyata,Daniel R. Radiloff,Jordana M. Henderson,Andrew Ujita,Omar O. Abudayyeh,Jonathan S. Gootenberg +27 more
TL;DR: Large sequences are integrated site specifically into the human genome without double-strand DNA cleavage, expanding the capabilities of genome editing by allowing large, multiplexed gene insertion without reliance on DNA repair pathways.
261
Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR
Kaiyi Jiang,Jeremy G. Koob,Xi Dawn Chen,Rohan N. Krajeski,Yifan Zhang,Verena Volf,Wenyuan Zhou,Samantha R Sgrizzi,Lukas Villiger,Jonathan S. Gootenberg,Fei Chen,Omar O. Abudayyeh +11 more
TL;DR: A programmable RNA-sensing technology, reprogrammable ADAR sensors (RADARS), which harnesses RNA editing by adenosine deaminases acting on RNA (ADAR) to gate translation of a cargo protein by the presence of endogenous RNA transcripts.
61
RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease
Kazuki Kato,Sae Okazaki,Cian Schmitt-Ulms,Kaiyi Jiang,Wenyuan Zhou,Junichiro Ishikawa,Yukari Isayama,Shungo Adachi,Tomohiro Nishizawa,Kira S. Makarova,Eugene V. Koonin,Omar O. Abudayyeh,Jonathan S. Gootenberg,Hiroshi Nishimasu +13 more
TL;DR: The type III-E CRISPR-Cas7-11-crRNA-Csx29 complex with and without target RNA (tgRNA) binding induces conformational changes in Csx29 as mentioned in this paper .
36
Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses
Kaiyi Jiang,Justin K. Lim,Samantha R Sgrizzi,Michael Trinh,Alisan Kayabolen,Natalya Yutin,Weidong Bao,Kazuki Kato,Eugene V. Koonin,Jonathan S. Gootenberg,Omar O. Abudayyeh +10 more
TL;DR: It is demonstrated that Fanzors can be harnessed for genome editing in human cells, highlighting the potential of these widespread eukaryotic RNA-guided nucleases for biotechnology applications.
29
RNA-triggered protein cleavage and cell death by the RNA-guided type III-E CRISPR-Cas nuclease-protease complex
Kazuki Kato,Sae Okazaki,Cian Schmitt-Ulms,Kaiyi Jiang,Wenyuan Zhou,Junichiro Ishikawa,Yukari Isayama,Shungo Adachi,Tomohiro Nishizawa,Kira S. Makarova,Eugene V. Koonin,Omar O. Abudayyeh,Jonathan S. Gootenberg,Hiroshi Nishimasu +13 more
TL;DR: Cryo-electron microscopy structures of the Cas7-11–crRNA–Csx29 complex with and without target RNA are reported, and it is demonstrated that target RNA binding induces a conformational change in Csx29 and results in the protease activation.