51 Papers
215 Citations
Kai Nie is an academic researcher from Chinese Center for Disease Control and Prevention. The author has contributed to research in topics: Medicine & Virus. The author has an hindex of 14, co-authored 35 publications. Previous affiliations of Kai Nie include National Health and Family Planning Commission & South China University of Technology.
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Papers
VIP: an integrated pipeline for metagenomics of virus identification and discovery.
TL;DR: VIP (“Virus Identification Pipeline”), a one-touch computational pipeline for virus identification and discovery from metagenomic NGS data, has been described and validated the feasibility and veracity of this pipeline with sequencing results of various types of clinical samples and public datasets.
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Visual Detection of High-Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye
TL;DR: This qualitative and colorimetric LAMP assay has potential usefulness for the rapid screening of HPV genotype 16, 18, 45, 52, and 58 infections, especially in resource-limited hospitals or rural clinics of provincial and municipal regions in China.
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Improved Detection Limit in Rapid Detection of Human Enterovirus 71 and Coxsackievirus A16 by a Novel Reverse Transcription–Isothermal Multiple-Self-Matching-Initiated Amplification Assay
TL;DR: In this paper, a reverse transcription-isothermal multiple-self-matching-initiated amplification (RT-IMSA) was proposed for the rapid detection of human enterovirus 71 (EV71) and coxsackievirus A16 (CVA16).
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Visual detection of human infection with influenza A (H7N9) virus by subtype-specific reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye.
Kai Nie,Xiang Zhao,Xiong Ding,Xiong Ding,Xiaodan Li,Shumei Zou,Junfeng Guo,Dayan Wang,Rongbao Gao,Xiyan Li,Weijuan Huang,Yue Long Shu,Xuejun Ma +12 more
TL;DR: The detection limits of both subtype-specific assays were comparable to those of validated H7 and N9 real-time PCR assays respectively and no cross-detection was observed with influenza A pandemic H1N1, H3N2, H5N 1, H9N2 or influenza B virus.
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TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus
Nan Shao,Fan Li,Kai Nie,Shi Hong Fu,Weijia Zhang,Weijia Zhang,Ying He,Wen Wen Lei,Qianying Wang,Guo Dong Liang,Yu Xi Cao,Huan Yu Wang +11 more
TL;DR: A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.
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