7 Papers
5 Citations
Kai Li is an academic researcher from University of South China. The author has contributed to research in topics: CRISPR & Mutant. The author has an hindex of 3, co-authored 7 publications.
Chat about Author
Papers
Improved EGFR mutation detection sensitivity after enrichment by Cas9/sgRNA digestion and PCR amplification.
Li Wang,Li Wang,Jing Jiang,Xiangning Li,Kai Li,Rongzhang He,Jia Li,Lili Duan,Weihao Luo,Zheng Hu,Zheng Hu,Dixian Luo,Dixian Luo +12 more
TL;DR: Results showed that after CRISPR/Cas9 enrichment, a low concentration of mutant DNA fragments could be detected by Sanger sequencing, which represented a 1000-fold increase compared with the untreated samples, indicating that this method has the potential to detect low copy number mutations at the early stage.
7
Identification of a Novel Cleavage Site and Confirmation of the Effectiveness of NgAgo Gene Editing on RNA Targets
Jiayao Qu,Jiayao Qu,Jiayao Qu,Yali Xie,Zhaoyi Guo,Zhaoyi Guo,Zhaoyi Guo,Xiangting Liu,Jing Jiang,Ting Chen,Kai Li,Zheng Hu,Zheng Hu,Dixian Luo +13 more
TL;DR: In this paper, an in vitro RNA cleavage system was designed and the cleavage site was verified by sequencing and the results confirmed that NgAgo-gDNA cleaves RNA not DNA.
7
Patent
Primer pair for detecting KRAS hot-spot mutation by utilizing blue-white screening
Cuilan Zhou,Peng Cuiying,Li Wen,Xiao Li,Kai Li +4 more
- 02 Dec 2015
TL;DR: In this paper, a primer pair for detecting hot-spot mutation of tumor mutant KRAS genes at the amino acid codons 12 and 13 was provided, where the principle of in-vitro adjustment of the reading frame of genes is adopted, and the blue-white screening for sub-cloning is used for detecting the hot spot mutation.
3
Recent progress in technological improvement and biomedical applications of the clustered regularly interspaced short palindromic repeats/cas system
Yanlan Li,Zheng Hu,Yufang Yin,Rongzhang He,Jian Hu,Weihao Luo,Jia Li,Gebo Wen,Li Xiao,Kai Li,Duan-Fang Liao,Dixian Luo +11 more
TL;DR: The improvements of CRISPR-Cas9 gene editing methods are introduced, the recent advances of this technology are summarized, and the biomedical applications of the technology are to edit not only cells but also embryos in clinical settings.
1
Patent
Method for rapidly preparing sgRNA by PCR with multiple overlap primers
Dixian Luo,Hu Zheng,Li Wang,Kai Li +3 more
- 15 Jan 2019
TL;DR: In this article, a plurality of overlapping primers was used to synthesize DNA template of sgRNA in one step for the first time, and the experiment proves that the DNA template has high cleavage efficiency.
1