Junichi Kaneshiro
RIKEN Quantitative Biology Center
7 Papers
26 Citations
Junichi Kaneshiro is an academic researcher from RIKEN Quantitative Biology Center. The author has contributed to research in topics: Protein crystallization & Polarization Microscopy. The author has an hindex of 4, co-authored 7 publications.
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Papers
Advanced CUBIC tissue clearing for whole-organ cell profiling.
Katsuhiko Matsumoto,Tomoki T Mitani,Shuhei A. Horiguchi,Junichi Kaneshiro,Tatsuya C. Murakami,Tomoyuki Mano,Hiroshi Fujishima,Ayumu Konno,Tomonobu M. Watanabe,Hirokazu Hirai,Hiroki R. Ueda +10 more
TL;DR: This protocol enables preparation of high-transparency organs that retain fluorescent protein signals within 7–21 d by immersion in CUBIC reagents, and allows analysis of numerous samples, providing a platform for next-generation biomedical research.
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Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy
TL;DR: The theoretical framework involves possible distortions of the polarization state due to optical elements between the polarization controller and measurement point and presents a mechanism for pre-compensating the polarization distortion.
18
Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots.
Taishi Kakizuka,Keigo Ikezaki,Junichi Kaneshiro,Hideaki Fujita,Tomonobu M. Watanabe,Taro Ichimura +5 more
TL;DR: Simultaneous nanometric tracking of multiple motor proteins was achieved by combining multicolor fluorescent labeling of target proteins and imaging spectroscopy, revealing dynamic behaviors of several motor proteins at the sub-diffraction-limit scale.
11
Second harmonic generation polarization microscopy as a tool for protein structure analysis
Junichi Kaneshiro,Yasushi Okada,Tomohiro Shima,Mika Tsujii,Katsumi Imada,Taro Ichimura,Taro Ichimura,Tomonobu M. Watanabe +7 more
TL;DR: It is demonstrated that a conformational change as well as its dynamics in protein macromolecular assemblies can be detected by means of SHG polarization measurement, and the potential of this method for protein structure analysis in physiological solutions at room temperature without any labeling is illustrated.
9
Second harmonic generation polarization microscopy as a tool for protein structure analysis
Junichi Kaneshiro,Yasushi Okada,Tomohiro Shima,Mika Tsujii,Katsumi Imada,Taro Ichimura,Taro Ichimura,Tomonobu M. Watanabe +7 more
TL;DR: Polarization measurement for second-harmonic generation is reported for the detection of protein conformational changes in solutions of macromolecular protein assemblies such as microtubules and protein crystals.