Junhao Yang
Celgene
13 Papers
80 Citations
Junhao Yang is an academic researcher from Celgene. The author has contributed to research in topics: Cell-free protein synthesis & Antigen. The author has an hindex of 9, co-authored 13 publications.
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Papers
Microscale to Manufacturing Scale-up of Cell-Free Cytokine Production—A New Approach for Shortening Protein Production Development Timelines
James Zawada,Gang Yin,Alexander R. Steiner,Junhao Yang,Alpana Naresh,Sushmita Mimi Roy,Daniel Gold,Henry Heinsohn,Christopher J. Murray +8 more
TL;DR: It is shown how a fully bioactive protein produced by OCFS from optimized frozen extract can be purified directly using a streamlined purification process that yields a biologically active cytokine, human granulocyte‐macrophage colony‐stimulating factor, produced at titers of 700 mg/L in 10 h.
419
Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system.
Yiren Xu,John Lee,Cuong Tran,Tyler H. Heibeck,Willie D. Wang,Junhao Yang,Ryan Stafford,Alexander R. Steiner,Aaron K. Sato,Trevor J. Hallam,Gang Yin +10 more
TL;DR: The use of a cell-free expression system (Xpress CF) to produce KIH bispecific antibodies in multiple scaffolds, including 2-armed heterodimeric scFv-KIH and one-armed asymmetric BiTE-KIh with tandem scFV are described.
88
Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins
Dan Groff,Stephanie Armstrong,Patrick Rivers,Juan Zhang,Junhao Yang,Evan Green,James Rozzelle,Shengwen Liang,Joseph D. Kittle,Alexander R. Steiner,Ramesh Baliga,Christopher D. Thanos,Trevor J. Hallam,Aaron K. Sato,Alice Yam +14 more
TL;DR: A protein additive screen was undertook to identify chaperone proteins that improve the folding and assembly of trastuzumab in OCFS and identified the disulfide isomerase DsbC and the prolyl isomerases FkpA as important positive effectors of IgG folding.
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Patent
Anti-pd-1 antibodies, compositions comprising anti-pd-1 antibodies and methods of using anti-pd-1 antibodies
Alice Yam,Ryan Stafford,Aaron Sato,John Lee,Avinash Gill,Junhao Yang,Heather Stephenson +6 more
- 10 Nov 2015
TL;DR: In this paper, the authors describe antibodies that selectively bind to PD-1 and its isoforms and homologs, and compositions comprising the antibodies, and methods of using the antibodies such as therapeutic and diagnostic methods.
39
Patent
Anti-cd47 antibodies and uses thereof
Aaron K. Sato,Ryan Stafford,Junhao Yang +2 more
- 28 Dec 2015
TL;DR: In this paper, compositions, methods and uses involving antibodies that specifically bind to human CD47 are presented, as well as uses and methods, such as therapeutic methods, diagnostic methods, and methods of making such antibodies.
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