Junfeng Xu
4 Papers
27 Citations
Junfeng Xu is an academic researcher. The author has contributed to research in topics: Signal & Nucleic acid quantitation. The author has an hindex of 1, co-authored 4 publications.
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Papers
Versatile detection with CRISPR/Cas system from applications to challenges
TL;DR: In this review, the characteristics of four commonly used Cas effectors are analyzed and detection strategies based on the binding activity and cleavage activity of CRISPR/Cas system are fully discussed.
97
Rotary Valve-Assisted Fluidic System Coupling with CRISPR/Cas12a for Fully Integrated Nucleic Acid Detection.
Hui Wu,Siwenjie Qian,Peng Cheng,Xiaofu Wang,Tingzhang Wang,Xiaoping Zhong,Yanju Chen,Qunqing Yang,Junfeng Xu,Jian Wu +9 more
TL;DR: In this article, a rotary valve-assisted fluidic chip coupled with CRISPR/Cas12a was established to achieve fully integrated nucleic acid detection, which achieved 3.1 × 101 copies of target DNA per reaction.
29
CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone.
Yanju Chen,Shuyue Wu,Hui Wu,Peng Cheng,Xiaofu Wang,Siwenjie Qian,Mengyao Zhang,Junfeng Xu,Feng Ji,Jian Wu +9 more
TL;DR: Wang et al. as discussed by the authors proposed a CRISPR-based checking method to solve gray zone problems, which provided a novel solution to eliminate gray zone sample problems with no additional effects on the PCR, which is operable and applicable in practical detection.
20
A reversible valve-assisted chip coupling with integrated sample treatment and CRISPR/Cas12a for visual detection of Vibrio parahaemolyticus.
Hui Wu,Yanju Chen,Qunqing Yang,Peng Cheng,Xiaofu Wang,Mengyao Zhang,Siwenjie Qian,Junfeng Xu,Jian Wu +8 more
TL;DR: Wang et al. as mentioned in this paper used a reversible valve-assisted chip coupled with CRISPR/Cas12a to detect Vibrio parahaemolyticus (V.para) in seafood.