Jun Kobayashi
8 Papers
Jun Kobayashi is an academic researcher. The author has contributed to research in topics: Femtosecond & M2 proton channel. The author has an hindex of 6, co-authored 7 publications.
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Papers
A three-dimensional movie of structural changes in bacteriorhodopsin
Eriko Nango,Antoine Royant,Antoine Royant,Minoru Kubo,Takanori Nakane,Cecilia Wickstrand,Tetsunari Kimura,Tomoyuki Tanaka,Kensuke Tono,Changyong Song,Rie Tanaka,Toshi Arima,Ayumi Yamashita,Jun Kobayashi,Toshiaki Hosaka,Eiichi Mizohata,Przemyslaw Nogly,Michihiro Sugahara,Daewoong Nam,Takashi Nomura,Tatsuro Shimamura,Dohyun Im,Takaaki Fujiwara,Yasuaki Yamanaka,Byeonghyun Jeon,Tomohiro Nishizawa,Tomohiro Nishizawa,Kazumasa Oda,Masahiro Fukuda,Rebecka Andersson,Petra Båth,Robert Dods,Jan Davidsson,Shigeru Matsuoka,Satoshi Kawatake,Michio Murata,Osamu Nureki,Shigeki Owada,Takashi Kameshima,Takaki Hatsui,Yasumasa Joti,Gebhard F. X. Schertler,Gebhard F. X. Schertler,Makina Yabashi,Ana-Nicoleta Bondar,Jörg Standfuss,Richard Neutze,So Iwata +47 more
TL;DR: Time-resolved serial femtosecond crystallography at an x-ray free electron laser shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.
XFEL structures of the influenza M2 proton channel: Room temperature water networks and insights into proton conduction
Jessica L. Thomaston,Rahel A. Woldeyes,Takanori Nakane,Ayumi Yamashita,Tomoyuki Tanaka,Kotaro Koiwai,Aaron S. Brewster,Benjamin A Barad,Yujie Chen,Thomas Lemmin,Monarin Uervirojnangkoorn,Toshi Arima,Jun Kobayashi,Tetsuya Masuda,Mamoru Suzuki,Michihiro Sugahara,Nicholas K. Sauter,Rie Tanaka,Osamu Nureki,Kensuke Tono,Yasumasa Joti,Eriko Nango,So Iwata,Fumiaki Yumoto,James S. Fraser,William F. DeGrado +25 more
TL;DR: High-resolution structures of the influenza M2 channel further the understanding of how protons are transported across a membrane bilayer through a constricted membrane protein environment and provides a graphical view of the response of water to a change in charge within a restricted channel environment.
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Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography
Fumitaka Mafuné,Ken Miyajima,Kensuke Tono,Yoshihiro Takeda,Jun-ya Kohno,Naoya Miyauchi,Jun Kobayashi,Yasumasa Joti,Eriko Nango,So Iwata,Makina Yabashi +10 more
TL;DR: Liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption and by finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements.
Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent
Takanori Nakane,Shinya Hanashima,Mamoru Suzuki,Haruka Saiki,Taichi Hayashi,Keisuke Kakinouchi,Shigeru Sugiyama,Satoshi Kawatake,Shigeru Matsuoka,Nobuaki Matsumori,Eriko Nango,Jun Kobayashi,Tatsuro Shimamura,Kanako Kimura,Chihiro Mori,Naoki Kunishima,Michihiro Sugahara,Yoko Takakyu,Shigeyuki Inoue,Tetsuya Masuda,Toshiaki Hosaka,Kensuke Tono,Yasumasa Joti,Takashi Kameshima,Takaki Hatsui,Makina Yabashi,Tsuyoshi Inoue,Osamu Nureki,So Iwata,Michio Murata,Eiichi Mizohata +30 more
TL;DR: The iodine-containing detergent provided strong anomalous and isomorphous difference signals, which enabled experimental phasing using lower-resolution reflections from fewer indexed images than phasing attempts reported previously, paving the way for de novo structure determination of membrane proteins, which often diffract poorly, even with the brightest XFEL beams.
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Ligand Recognition by the Lipid Transfer Domain of Human OSBP Is Important for Enterovirus Replication.
Jun Kobayashi,Minetaro Arita,Shota Sakai,Hirotatsu Kojima,Miki Senda,Toshiya Senda,Kentaro Hanada,Ryuichi Kato +7 more
TL;DR: Using the M446W variant as a functional replacement for the endogenous OSBP in the presence of T-00127-HEV2, previously unappreciated amino acid residues required for viral replication are identified.
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