Jörg Mütze
Dresden University of Technology
7 Papers
92 Citations
Jörg Mütze is an academic researcher from Dresden University of Technology. The author has contributed to research in topics: Fluorescence correlation spectroscopy & Fluorescence cross-correlation spectroscopy. The author has an hindex of 5, co-authored 7 publications.
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Papers
Fluorescence correlation spectroscopy in vivo
TL;DR: Flurescence Correlation Spectroscopy (FCS) is an optical thechnique used to accurately the dynamics, local concertration, and photophysics of single molecules both in vitro and vivo as mentioned in this paper.
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Fluorescence cross-correlation spectroscopy reveals mechanistic insights into the effect of 2'-O-methyl modified siRNAs in living cells.
TL;DR: Two new fluorescence cross-correlation spectroscopy assays resolve different steps and stages of RISC assembly and target recognition with heretofore unresolved detail in living cells, which is needed to develop therapeutic siRNAs with optimized in vivo properties.
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Independence of Maximum Single Molecule Fluorescence Count Rate on the Temporal and Spectral Laser Pulse Width in Two-Photon FCS
TL;DR: This observation confirms the current working hypothesis for two-photon fluorescence correlation spectroscopy that bleaching and saturation, and thus, the inherent properties of the dye system, are the dominant effects limiting the quality of measurements.
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Fluorescence lifetime correlation spectroscopy for precise concentration detection in vivo by background subtraction
Maria Gärtner,Jörg Mütze,Thomas Ohrt,Petra Schwille +3 more
- 14 Jun 2009
TL;DR: In this paper, the authors used the fluorescent lifetime of the different contributions to distinguish between the fluorescent signal and the autofluorescent background in vivo in a single measurement, and showed that it is possible to distinguish the fluorescent signals from the background in the presence of background noise.
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In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy
Jörg Mütze,Thomas Ohrt,Zdeněk Petrášek,Petra Schwille +3 more
- 01 Jan 2010
TL;DR: An EGFP based cell line that allows to study the dynamics and localization of the RNA-induced silencing complex (RISC) with FCS in vivo is created, which has so far been inaccessible with other experimental methods.
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