The widespread evolution of resistance in rigid ryegrass populations to the highly effective, in-crop, selective herbicides used within southern Australian grain-crop production systems has severely diminished the available herbicide resource. A new PRE grass-selective herbicide, pyroxasulfone, may offer Australian grain producers a new option for rigid ryegrass control in wheat crops. The efficacy and level of selectivity of rigid ryegrass control with pyroxasulfone was investigated for a range of annual crop species in potted-plant, dose–response studies. In comparison with other currently available PRE herbicides, pyroxasulfone provided effective control of both resistant and susceptible rigid ryegrass populations. Additionally, control of these populations was achieved at rates that had little or no effect on the growth and survival of wheat. This crop was also the most tolerant of cereal species, with triticale, barley, and oat being more injured at higher pyroxasulfone rates than wheat was....

/pdf/the-potential-for-pyroxasulfone-to-selectively-control-1d56ydbrqs.pdf

The Potential for Pyroxasulfone to Selectively Control Resistant and Susceptible Rigid Ryegrass (Lolium rigidum) Biotypes in Australian Grain Crop Production Systems

BACKGROUND
A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors for restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).

RESULTS
Enhanced metabolism via cytochrome P450 enzymes is the mechanism of resistance in HPPD-R. Amitrole partially restored the activity of mesotrione, whereas malathion, amitrole, and piperonyl butoxide restored the activity of tembotrione and topramezone in HPPD-R. While corn was injured through malathion followed by mesotrione application a week after treatment, the injury was transient, and the crop recovered.

CONCLUSION
The use of cytochrome P450 inhibitors with tembotrione may provide a new way for controlling HPPD-inhibitor resistant A. tuberculatus, but further research is needed to identify the cytochrome P450 candidate gene(s) conferring metabolism-based resistance. The results presented here aid to gain an insight into non-target-site resistance weed management strategies.

/pdf/reversing-resistance-to-tembotrione-in-an-amaranthus-36beijcyc1.pdf

Reversing resistance to tembotrione in an Amaranthus tuberculatus (var. rudis) population from Nebraska, USA with cytochrome P450 inhibitors.

Since weed management is such a critical component of agronomic crop production systems, herbicides are widely used to provide weed control to ensure that yields are maximized. In the last few years, herbicide-resistant (HR) crops, particularly those that are glyphosate-resistant, and more recently, those with dicamba (3,6-dichloro-2-methoxybenzoic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid) resistance are changing the way many growers manage weeds. However, past reliance on glyphosate and mistakes made in stewardship of the glyphosate-resistant cropping system have directly led to the current weed resistance problems that now occur in many agronomic cropping systems, and new technologies must be well-stewarded. New herbicide-resistant trait technologies in soybean, such as dicamba-, 2,4-D-, and isoxaflutole- ((5-cyclopropyl-4-isoxazolyl)[2-(methylsulfonyl)-4-(trifluoromethyl)phenyl]methanone) resistance, are being combined with glyphosate- and glufosinate-resistance traits to manage herbicide-resistant weed populations. In cropping systems with glyphosate-resistant weed species, these new trait options may provide effective weed management tools, although there may be increased risk of off-target movement and susceptible plant damage with the use of some of these technologies. The use of diverse weed management practices to reduce the selection pressure for herbicide-resistant weed evolution is essential to preserve the utility of new traits. The use of herbicides with differing sites of action (SOAs), ideally in combination as mixtures, but also in rotation as part of a weed management program may slow the evolution of resistance in some cases. Increased selection pressure from the effects of some herbicide mixtures may lead to more cases of metabolic herbicide resistance. The most effective long-term approach for weed resistance management is the use of Integrated Weed Management (IWM) which may build the ecological complexity of the cropping system. Given the challenges in management of herbicide-resistant weeds, IWM will likely play a critical role in enhancing future food security for a growing global population.

https://www.mdpi.com/2077-0472/9/8/180/pdf

Emerging Challenges for Weed Management in Herbicide-Resistant Crops

A field experiment was conducted in Boone and Callaway counties in Missouri in 2010 and 2011 to investigate herbicide programs for the management of summer annual grass and broadleaf weeds in soybean resistant to 2,4-D and glufosinate. Results revealed that the addition of 0.56, 0.84, or 1.12 kg ha−1 2,4-D (amine) to either or both POST applications of glufosinate in a two-pass POST herbicide program increased control of common waterhemp compared to two POST applications of glufosinate alone. Similar levels of common cocklebur, giant foxtail, large crabgrass, and barnyardgrass control were achieved with any of the two-pass POST programs that contained 2,4-D compared to two-pass POST programs containing glufosinate alone. Similar control of these species was also achieved with the inclusion of 2,4-D in either the first or second pass of glufosinate. Two-pass programs resulted in the highest levels of weed control (90%). Annual grass and broadleaf weed control was as much as 59% lower when one-pass...

https://bioone.org/journals/weed-technology/volume-27/issue-1/WT-D-12-00099.1/Influence-of-Herbicide-Programs-on-Weed-Management-in-Soybean-with/10.1614/WT-D-12-00099.1.pdf

Influence of Herbicide Programs on Weed Management in Soybean with Resistance to Glufosinate and 2,4-D

Antibodies have proven to be central in the development of diagnostic methods over decades, moving from polyclonal antibodies to the milestone development of monoclonal antibodies. Although monoclonal antibodies play a valuable role in diagnosis, their production is technically demanding and can be expensive. The large size of monoclonal antibodies (150 kDa) makes their re-engineering using recombinant methods a challenge. Single-domain antibodies, such as "nanobodies," are a relatively new class of diagnostic probes that originated serendipitously during the assay of camel serum. The immune system of the camelid family (camels, llamas, and alpacas) has evolved uniquely to produce heavy-chain antibodies that contain a single monomeric variable antibody domain in a smaller functional unit of 12-15 kDa. Interestingly, the same biological phenomenon is observed in sharks. Since a single-domain antibody molecule is smaller than a conventional mammalian antibody, recombinant engineering and protein expression in vitro using bacterial production systems are much simpler. The entire gene encoding such an antibody can be cloned and expressed in vitro. Single-domain antibodies are very stable and heat-resistant, and hence do not require cold storage, especially when incorporated into a diagnostic kit. Their simple genetic structure allows easy re-engineering of the protein to introduce new antigen-binding characteristics or attach labels. Here, we review the applications of single-domain antibodies in laboratory diagnosis and discuss the future potential in this area.

Application of Single-Domain Antibodies ("Nanobodies") to Laboratory Diagnosis.

Field experiments were conducted in Nebraska with the experimental herbicide KIH-485 on soils with three different levels of organic matter (OM) to ascertain a dose response for weed control and corn tolerance. Dose–response curves based on the log-logistic model were used to determine the effective dose that provides 90% weed control (ED90 values) for three grasses (green foxtail, field sandbur, large crabgrass) and two broadleaf weeds (velvetleaf, tall waterhemp). The ED90 values for green foxtail control were 143, 165, and 202 g ai/ha for soils with 1, 2, and 3% OM, respectively at 28 d after treatment (DAT). The highest dose of 371 g ai/ha was needed for field sandbur control at 28 DAT, compared with 141 g ai/ha for large crabgrass, 152 g ai/ha for tall waterhemp, and 199 g ai/ha for velvetleaf. There was no significant corn injury observed. Grain yield increased with increasing doses of KIH-485; optimum yield was achieved at about 195 g ai/ha. From the dose–response curves it is clear that t...

Dose–Response Curves of Kih-485 for Preemergence Weed Control in Corn

Although glyphosate controls many plant species, certain broadleaf weeds in Nebraska's cropping systems exhibit various levels of tolerance to the labeled rates of this herbicide, including ivyleaf morningglory, Venice mallow, yellow sweetclover, common lambsquarters, velvetleaf, kochia, Russian thistle, and field bindweed. Therefore, two field studies were conducted in 2004 and 2005 at Concord and North Platte, NE, to evaluate performance of (1) seven preemergence (PRE) herbicides and (2) glyphosate tank mixes applied postemergence (POST) at three application times for control of eight weed species that are perceived as problem weeds in glyphosate-resistant soybean in Nebraska. The PRE herbicides, including sulfentrazone plus chlorimuron, pendimethalin plus imazethapyr, imazaquin, and pendimethalin plus imazethapyr plus imazaquin provided more than 85% control of most weed species tested in this study 28 d after treatment (DAT). However, sulfentrazone plus chlorimuron and pendimethalin plus imaz...

https://bioone.org/journals/weed-technology/volume-23/issue-4/WT-09-012.1/Problem-Weed-Control-in-Glyphosate-Resistant-Soybean-with-Glyphosate-Tank/10.1614/WT-09-012.1.pdf

Problem Weed Control in Glyphosate-Resistant Soybean with Glyphosate Tank Mixes and Soil-Applied Herbicides

Application timing and adjuvant type affected saflufenacil efficacy on selected broadleaf weeds

Tolerance of winter wheat (Triticum aestivum L.) to pre-emergence and post-emergence application of saflufenacil.

Received 25 Oct. 2018. Accepted 3 Dec. 2018. *Corresponding author (sknezevic2@unl.edu). ABSTRACT New dicamba-based herbicides such as Engenia (N,N-bis-(3-aminopropyl) methylamine salt) and XtendiMax (diglycolamine salt) with VaporGrip technology were developed to reduce dicamba volatility and drift; however, there are claims that these products can still volatilize or drift. Field studies were conducted to evaluate glyphosate (N-(phosphonomethyl)glycine))–resistant (GR) soybean response to micro-rates (0, 1/1000, 1/500, 1/100, 1/50, and 1/10 of the label rate, 560 g a.e. ha-1) of the two new dicamba products compared with Clarity (diglycolamine salt) applied at three growth stages. The GR soybean [Glycine max (L.) Merr.] was equally impacted by the micro-rates of all three products as measured by visual injury, height reduction, delayed physiological maturity, and yield reduction. The greatest visual injury (80%), plant height reduction (65%), maturity delay (22 d), and soybean yield loss (96%) was caused by 1/10 of the dicamba label rate when applied at V7/R1 soybean growth stage. In addition, estimation of effective dose for 5, 10, or 20% yield reduction suggested that V7/R1 was the most sensitive soybean growth stage to the three dicamba products. For example, 10% yield reduction occurred when 1.83 to 1.85 g a.e. ha-1 (~1/300 of the label rate) of Engenia was applied at V2 or R2, whereas, a lower dose of 0.32 g a.e. ha-1 (1/1750 of the label rate) of Engenia caused the same level of yield reduction when applied at V7/R1. Similar doses were estimated for Clarity and XtendiMax; therefore dicamba drift should be avoided at all costs, because GR soybean was equally sensitive to low rates of all three tested products with different formulations or technologies.

Glyphosate-Resistant Soybean Response to Micro-Rates of Three Dicamba-Based Herbicides

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