John Yochem
University of Oregon
5 Papers
25 Citations
John Yochem is an academic researcher from University of Oregon. The author has contributed to research in topics: Gene & Gene mapping. The author has an hindex of 5, co-authored 5 publications.
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Papers
A Survey of New Temperature-Sensitive, Embryonic-Lethal Mutations in C. elegans: 24 Alleles of Thirteen Genes
Sean M. O'Rourke,Clayton Carter,Luke Carter,Sara N. Christensen,Minh P. Jones,Bruce Nash,Meredith H. Price,Douglas W. Turnbull,Aleena R. Garner,Danielle R. Hamill,Valerie R. Osterberg,Rebecca Lyczak,Erin E. Madison,Michael H. Nguyen,Nathan A. Sandberg,Noushin Sedghi,John H. Willis,John Yochem,Eric A. Johnson,Bruce Bowerman +19 more
TL;DR: It is found that 62% of the mutants display additional phenotypes when shifted to the restrictive temperature as L1 larvae, in addition to causing embryonic lethality after L4 upshifts, and 13 out of the 24 mutations appear to be fast-acting, making them particularly useful for careful dissection of multiple essential requirements.
High-Throughput Cloning of Temperature-Sensitive Caenorhabditis elegans Mutants with Adult Syncytial Germline Membrane Architecture Defects
TL;DR: This work has used a forward genetic screen to identify 20 temperature-sensitive mutations in 19 essential genes that cause defects in the germline membrane architecture, and identified the causal mutations in 10 of these mutants.
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Rapid Mapping and Identification of Mutations in Caenorhabditis elegans by Restriction Site-Associated DNA Mapping and Genomic Interval Pull-Down Sequencing
Sean M. O'Rourke,John Yochem,Amy A. Connolly,Meredith H. Price,Luke Carter,Joshua B. Lowry,Douglass W. Turnbull,Nick Kamps-Hughes,Nicholas Stiffler,Michael R. Miller,Eric A. Johnson,Bruce Bowerman +11 more
TL;DR: Two genomic approaches for more rapidly determining the identity of the affected genes in Caenorhabditis elegans mutants are reported, including the use of restriction site-associated DNA (RAD) polymorphism markers for rapidly mapping mutations after chemical mutagenesis and mutant isolation.
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MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature Sensitive Mutants via Molecular Inversion Probes
Calvin Mok,Vinci Au,Owen Thompson,Mark L. Edgley,Louis Gevirtzman,John Yochem,Joshua B. Lowry,Nadin Memar,Matthew R. Wallenfang,Dominique Rasoloson,Bruce Bowerman,Ralf Schnabel,Geraldine Seydoux,Donald G. Moerman,Robert H. Waterston +14 more
TL;DR: The results suggest this collection of strains contains a diverse library of TS alleles for genes involved in development and reproduction, and the MIP-MAP protocol is a robust method to genetically map mutations in both viable and essential genes.
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MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature-Sensitive Mutants via Molecular Inversion Probes.
Calvin Mok,Vinci Au,Owen Thompson,Mark L. Edgley,Louis Gevirtzman,John Yochem,Joshua B. Lowry,Nadin Memar,Matthew R. Wallenfang,Dominique Rasoloson,Bruce Bowerman,Ralf Schnabel,Geraldine Seydoux,Donald G. Moerman,Robert H. Waterston +14 more
TL;DR: MIP-MAP is a robust method to genetically map mutations in both viable and essential genes and should be adaptable to other organisms and may also simplify tracking of individual genotypes within population mixtures.