John Sleep
King's College London
21 Papers
107 Citations
John Sleep is an academic researcher from King's College London. The author has contributed to research in topics: Myosin & Actin. The author has an hindex of 14, co-authored 21 publications. Previous affiliations of John Sleep include Randall Division of Cell and Molecular Biophysics.
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Papers
Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study.
TL;DR: The observations indicate that the elastic character is at least as much a consequence of the attachment of spectrin as of a continuous membrane-bound network, and they offer a rationale for formation of elliptocytes in genetic conditions associated with membrane-skeletal perturbations.
284
In vitro motility speed of slow myosin extracted from single soleus fibres from young and old rats
TL;DR: It is concluded that an age‐related alteration in myosin contributes to the slowing of the maximum shortening velocity (V0) observed in soleus muscle fibres expressing the β/slow MyHC isoform.
98
Mechanokinetics of Rapid Tension Recovery in Muscle: The Myosin Working Stroke Is Followed by a Slower Release of Phosphate
David Aitchison Smith,John Sleep +1 more
TL;DR: A version of the theory of A. F. Huxley and R. M. Simmons for the length-step response is averaged over elastic strains imposed by filament structure and extended to include a Pi-release transition, which provides a better fit for theories of muscle contraction.
57
The working stroke upon myosin–nucleotide complexes binding to actin
TL;DR: It is concluded that myosin, while dissociated from actin, must be able to take up at least two mechanical conformations and results are consistent with these conformations corresponding to the two states characterized at high resolution, which are commonly referred to in terms of having open and closed nucleotide binding pockets.
32
Strain-dependent kinetics of the myosin working stroke, and how they could be probed with optical-trap experiments.
David Aitchison Smith,John Sleep +1 more
TL;DR: It is predicted that the two kinds of averaging give diverging estimates of the working stroke as the resolving time of the event detector is decreased to 1 ms or less, the discrepancy being critically dependent on the strain dependence of the stroke rate.
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