John C. Williams
Stony Brook University
16 Papers
68 Citations
John C. Williams is an academic researcher from Stony Brook University. The author has contributed to research in topics: Optogenetics & Channelrhodopsin. The author has an hindex of 7, co-authored 16 publications.
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Papers
Computational optogenetics: empirically-derived voltage- and light-sensitive channelrhodopsin-2 model.
John C. Williams,Jianjin Xu,Zhongju Lu,Aleksandra Klimas,Xuxin Chen,Christina M. Ambrosi,Ira S. Cohen,Emilia Entcheva +7 more
TL;DR: The ability of ChR2 to trigger action potentials in human cardiomyocytes at relatively low light levels is demonstrated, as well as the differential response of these cells to light, with the Purkinje cells being most easily excitable and ventricular cells requiring the highest irradiance at all pulse durations.
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A comprehensive multiscale framework for simulating optogenetics in the heart
TL;DR: A comprehensive framework for multi-scale modelling of cardiac optogenetics is presented, allowing both mechanistic examination of optical control and exploration of potential therapeutic applications, and making possible the prediction of emergent behaviour resulting from interactions at sub-organ scales.
OptoDyCE: Automated System for High-Throughput All-Optical Dynamic Cardiac Electrophysiology
Aleksandra Klimas,Jinzhu Yu,Christina M. Ambrosi,John C. Williams,Harold Bien,Emilia Entcheva +5 more
TL;DR: OptoDyCE is implemented and validated, a fully automated system for all-optical cardiac electrophysiology that provides high-throughput means of cellular interrogation, i.e. allows for dynamic testing of >600 multicellular samples or compounds per hour, and yields high-content information about the action of a drug over time, space and doses.
Optogenetic versus Electrical Stimulation of Human Cardiomyocytes: Modeling Insights
John C. Williams,Emilia Entcheva +1 more
TL;DR: It is revealed that direct electrical current injection (rectangular pulse) is more efficient at short pulses, whereas voltage-mediated negative feedback leads to self-termination of ChR2 current and renders optical stimulation more efficient for long low-intensity pulses.
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Channelrhodopsin2 Current During the Action Potential: “Optical AP Clamp” and Approximation
Emilia Entcheva,John C. Williams +1 more
TL;DR: An “optical AP clamp” and its approximation employing measured current-voltage curve for ChR2 are discussed, applicable to voltage- and light-sensitive ion currents operating in excitable cells, e.g. cardiomyocytes or neurons.