John B. Warner
École Polytechnique Fédérale de Lausanne
6 Papers
John B. Warner is an academic researcher from École Polytechnique Fédérale de Lausanne. The author has contributed to research in topics: Huntingtin & Fusion protein. The author has an hindex of 4, co-authored 6 publications.
Chat about Author
Papers
Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths.
John B. Warner,Kiersten M. Ruff,Piau Siong Tan,Edward A. Lemke,Rohit V. Pappu,Hilal A. Lashuel +5 more
TL;DR: It is shown that monomeric Httex1 adopts tadpole-like architectures for polyQ lengths below and above the pathological threshold, which suggests that higher order homotypic and/or heterotypic interactions within distinct sub-populations of neurons are likely to be the main source of sharp polyQ length dependencies of HD.
100
An Intein-based Strategy for the Production of Tag-free Huntingtin Exon 1 Proteins Enables New Insights into the Polyglutamine Dependence of Httex1 Aggregation and Fibril Formation
TL;DR: This work reports an intein-based strategy that allows, for the first time, the rapid and efficient production of native tag-free Httex1 with polyQ repeats ranging from 7Q to 49Q, and reveals the inability of Ht Tex1–7Q/15Q to undergo amyloid fibril formation and an inverse correlation between fibrils length and polyQ repeat length.
40
Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington's disease.
Ya Zhou,Thomas R. Peskett,Thomas R. Peskett,Christian Landles,John B. Warner,Kirupa Sathasivam,Edward J Smith,Shu Chen,Ronald Wetzel,Hilal A. Lashuel,Gillian P. Bates,Helen R. Saibil +11 more
TL;DR: In this paper, the authors show that mutant exon 1 HTT proteins are recruited to a subset of cytoplasmic aggregates in the cell bodies of neurons in brain sections from presymptomatic mice, but not wild-type, mice.
Mid-infrared plasmonic nanoantennas for protein structure detection
Dordaneh Etezadi,John B. Warner,Francesco Simone Ruggeri,Giovanni Dietler,Hilal A. Lashuel,Hatice Altug +5 more
- 25 Jun 2017
TL;DR: In this paper, a plasmonic mid-IR nanoantennas can sensitively differentiate the secondary structural compositions of protein monolayers both in dry and aqueous environments.
Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection
Dordaneh Etezadi,John B. Warner,Francesco Simone Ruggeri,Francesco Simone Ruggeri,Giovanni Dietler,Hilal A. Lashuel,Hatice Altug +6 more
TL;DR: This work introduces a non-invasive, label-free mid-IR nanoantenna-array sensor for secondary structure identification of nanometer-thin protein layers in aqueous solution by resolving the content of plasmonically enhanced amide I signatures.