The use of potential biostimulants is of broad interest in plant science for improving yields The application of a humic derivative called fulvic acid (FA) may improve forage crop production FA is an uncharacterized mixture of chemicals and, although it has been reported to increase growth parameters in many species including legumes, its mode of action remains unclear Previous studies of the action of FA have lacked appropriate controls, and few have included field trials Here we report yield increases due to FA application in three European Medicago sativa cultivars, in studies which include the appropriate nutritional controls which hitherto have not been used No significant growth stimulation was seen after FA treatment in grass species in this study at the treatment rate tested Direct application to bacteria increased Rhizobium growth and, in M sativa trials, root nodulation was stimulated RNA transcriptional analysis of FA-treated plants revealed up-regulation of many important early nodulation signalling genes after only 3 d Experiments in plate, glasshouse, and field environments showed yield increases, providing substantial evidence for the use of FA to benefit M sativa forage production

/pdf/fulvic-acid-increases-forage-legume-growth-inducing-2znx3dhuur.pdf

Fulvic acid increases forage legume growth inducing preferential up-regulation of nodulation and signalling-related genes.

Chayote (Sechium edule), a member of the Cucurbitaceae family, is cultivated throughout tropical and subtropical regions of the world and utilized in pharmaceutical, cosmetic and food industries because it is an excellent source of minerals, dietary fibers, protein, vitamins, carotenoids, polysaccharides, phenolic and flavonoid compounds, and other nutrients. Chayote extracts process various medicinal properties, such as anti-cardiovascular, antidiabetic, antiobesity, antiulcer, and anticancer properties. With the rapid advancements of molecular biology and sequencing technology, studies on chayote have been carried out. Research advances, including molecular makers, breeding, genomic research, chemical composition, and pests and diseases, regarding chayote are reviewed in this paper. Future exploration and application trends are briefly described. This review provides a reference for basic and applied research on chayote, an important Cucurbitaceae vegetable crop.

/pdf/origin-evolution-breeding-and-omics-of-chayote-an-important-20z40hrpt2.pdf

Origin, Evolution, Breeding, and Omics of Chayote, an Important Cucurbitaceae Vegetable Crop.

Pseudomonas sp. TK35-L enhances tobacco root development and growth by inducing HRGPnt3 expression in plant lateral root formation

The ability of rhizobacteria to compete with other microorganisms for root colonization may be critical for its establishment on a root. Over a 6 day period, visualization of the spatial and temporal rhizosphere distribution of a bioluminescent-marked rhizobacterium, Pseudomonas putida, strain GR7.4lux, was examined on soybean grown in non-sterile soil conditions. Luminometry technologies showed a rapid root distribution of rhizobacteria where bioluminescence was particularly intense on the seed and upper root parts. The results provide new information on rhizobial root distribution, where, using enrichment broth, 50% of the root tips were still colonized by rhizobacteria up to 6 days after sowing. This suggests that rhizobial enrichment is required to detect low populations at the root tip. Bioluminescent technology represents a promising alternative to previous methods for studying rhizobial growth and distribution on roots.

Spatial and temporal distribution of a bioluminescent‐marked Pseudomonas putida on soybean root

The objective of the work was to isolate and screened for phosphate solubilization, multiple plant growth promoting traits and phytopathogenic antagonism from chilli rhizosphere .The isolate putatively identified as Bacillus endophyticus AVP9 on the basis of biochemical characteristics and 16srRNA partial gene sequence analysis. Acid phosphatase activity of AVP9 was also characterized for the first time and its activity in correlation with growth was studied at different PH (3.0 to 4.8), 12 different amino acids and 15 carbon sources After growth optimization Bacillus endophyticus AVP9 showed 1112ppm inorganic phosphate solubilization and multiple plant growth promoting traits indole acetic acid(80µg), ammonia (110 µg) , HCN ,catalase and siderophore production . Maximum acid phosphatase activity was found at PH 3.4(0.2IU), Fructose (0.86IU) and Mannitol (0.83IU) and no correlation was observed with growth. Multiple potential of isolate AVP9 can be exploited in agricultural and industrial applications as a promising microorganism.

/pdf/fluorescent-bacillus-endophyticus-avp9-multiple-potential-1uv0qoqbc4.pdf

Fluorescent Bacillus endophyticus AVP9-Multiple potential for phosphate solubilization, plant growth promotion and bio control†

There is a lack of knowledge concerning the effect of nitrate-nitrogen (NO3(-)-N) at levels known to inhibit nodule formation and functioning on root colonization of dinitrogen-fixing legumes. Firstly, this study investigated potential differences between Rhizobium leguminosarum bv. viciae 175F9 and its bioluminescent-labeled strain 175F9.lux on root colonization of faba bean (Vicia faba L.) and pea (Pisum sativum L.). These two strains similarly colonized the roots of both hosts. Secondly, this study evaluated the effects of 0 and 10 mol x m(-3) NO3(-)-N on root colonization of faba bean and pea by strain 175F9.lux, over time. Averaged over both hosts and harvest dates, the presence of NO3(-)-N increased the rhizobial population and the root length colonized. In addition, our results showed that bioluminescence activity increased from 7 to 14 days after sowing and was not correlated to rhizobial population. Finally, to demonstrate that an increase in bioluminescence activity was not an indirect effect of nitrate on R. leguminosarum bv. viciae 175F9.lux, this study investigated the effects of increasing carbon (mannitol) and nitrogen (NO3(-)-N) concentrations on the rhizobial population and bioluminescence activity. The carbon source was more important than the nitrogen source to increase the rhizobial population and bioluminescence activity, which increased with increasing mannitol concentration, but not with increasing nitrate concentration. Results from this study demonstrated that NO3(-)-N increased rhizobial population, especially for faba bean, and the length of root colonized.

Root colonization of faba bean (Vicia faba L.) and pea (Pisum sativum L.) by Rhizobium leguminosarum bv. viciae in the presence of nitrate-nitrogen.

/pdf/movement-of-genetically-engineered-xanthomonas-campestris-pv-25vsdm2aqr.pdf

Movement of genetically engineered xanthomonas campestris pv. campestris in the environment

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Root colonization of faba bean (Vicia faba L.) and pea (Pisum sativum L.) by Rhizobium leguminosarum bv. viciae in the presence of nitrate-nitrogen.

Movement of genetically engineered xanthomonas campestris pv. campestris in the environment