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The invention discloses a method for determining microcystin MC-LR in a water body, which belongs to the technical field of environmental protection The method for determining microcystin MC-LR in the water body utilizes glass fiber (GF)/ C glass fiber filter paper to filter frustules in a water sample to be determined, and a solid-phase extraction column-SPE column which takes C18 silica gel as a filler to separate and purify the filtered water sample to be determined, a high performance liquid chromatography is used to detect, and then, the pre-obtained standard curves of the corresponding peak areas of microcystin MC-LR standard substances with different concentrations is utilized to calculate the content of the microcystin MC-LR in the water sample to be determined The method for determining microcystin MC-LR in the water body has the advantages of simplicity in steps, convenience in operation, reduction in eluant usage and environmental toxicity, safety in operating personnel, low detection cost and the like, and the minimum detection limit is 0002mg/L

Method for determining microcystin MC-LR in water body

The global usage of veterinary antibiotics is significant. Antibiotics can be released into aquatic environments and elicit toxic effects on non-target organisms. In this study, the growth characteristics and toxin release of the cyanobacterium Microcystis aeruginosa (M. aeruginosa) were examined to investigate the physiological effects of tetracycline antibiotics on aquatic life. Results showed that the degree of toxicities of the following target antibiotics was TC (tetracycline hydrochloride) > CTC (chlortetracycline hydrochloride) > OTC (oxytetracycline hydrochloride) in terms of growth parameters, EC10 (0.63, 1.86, and 3.02 mg/L, respectively), and EC20 (1.58, 4.09, and 4.86 mg/L, respectively) values. These antibiotics inhibited the production of microcystin-LR (MC-LR) to varying degrees. CTC interfered M. aeruginosa cells and decreased their ability to release MC-LR, but this antibiotic stimulated the ability of these cells to synthesize MC-LR at 2 and 5 mg/L. OTC elicited a relatively weaker toxicity than CTC did and reduced MC-LR release. TC was the most toxic among the three antibiotics, and this antibiotic simultaneously reduced intracellular and extracellular MC-LR equivalents. Our results helped elucidate the effects of tetracycline antibiotics on M. aeruginosa, which is essential for environmental evaluation and protection. Our results are also helpful for guiding the application of veterinary antibiotics in agricultural settings.

Toxin Release of Cyanobacterium Microcystis aeruginosa after Exposure to Typical Tetracycline Antibiotic Contaminants.

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Method for determining microcystin MC-LR in water body

Toxin Release of Cyanobacterium Microcystis aeruginosa after Exposure to Typical Tetracycline Antibiotic Contaminants.