This study established a new method for regenerating Anthurium andraeanum Lind. and evaluated effects of different wavelengths from light-emitting diodes (LEDs) on rooting and growth of adventitious shoots. Callus occurred in leaf explants of A. andraeanum 'Alabama' and 'Sierra' cultured on a modified Murashige and Skoog (MS) basal medium supplemented with four concentrations of N-phenyl-N#-1,2,3-thiadiazol-5- ylurea (TDZ). Adventitious shoots were induced from callus pieces (' 1c m 3 ) cultured on the modified MS medium containing 6-benzyladenine (BA) with kinetin (KN), BA, and/or KN with 3-indolebutyric acid (IBA) or a-naphthalene acetic acid (NAA). Results showed that 1.82 mM TDZ induced 83.3% and 77.8% of leaf explants of 'Alabama' and 'Sierra' to produce callus and 24.9 and 24.7 adventitious shoots were produced per callus piece of 'Alabama' and 'Sierra' cultured on the modified MS medium containing 0.89 m MB A, 2.32 mM KN, and 0.98 mM IBA, respectively. Adventitious shoots were cut and rooted in the modified MS medium containing 0.98 mM IBA and grown under the same light level but with different light qualities. All adventitious shoots rooted; root numbers, root lengths, root fresh and dry weights, and leaf area of plantlets grown under red plus blue light were comparable to those grown under conventional fluorescent white light. Shoot height was the greatest in monochromic blue light followed by red light. Shoot fresh and dry weights of plantlets grown under red plus blue light, however, were significantly greater than those grown under the other light qualities. Plantlets grown under red plus blue light had 22.7% greater total dry weight and more balanced root-to-shoot ratio than those grown under fluorescent white light. These results suggested the use of complex of red plus blue LED could be an option for improving growth of A. andraeanum plantlets in vitro.

/pdf/regeneration-of-anthurium-andraeanum-from-leaf-explants-and-457cbpwu9t.pdf

Regeneration of Anthurium andraeanum from Leaf Explants and Evaluation of Microcutting Rooting and Growth under Different Light Qualities

Anthurium in vitro: A review

A successful protocol for high frequency callus induction and plant regeneration from Anthurium andreanum Linden ex Andre cv. Tropical half-anthers is described. Different variables using Winarto and Teixeira and Murashige and Skoog basal media supplemented with several plant growth regulators [2,4-dichlorophenoxy acetic acid (0.1–1.0 mg/l), α-naphthalene acetic acid (0.01–0.2 mg/l), thidiazuron (0.5–2.0 mg/l), 6-benzylaminopurine (0.5–1.0 mg/l), and kinetin (0.5–1.0 mg/l)] were tested for their ability to induce high frequency callusing in half-anthers, indirect regeneration and rooting of shoots. Basal medium, as well as the combination and concentration of hormones applied, had a significant effect on callus formation, shoot regeneration and adventitious root formation. Winarto and Teixeira-1, an original basal medium containing 0.01 mg/l α-naphthalene acetic acid, 0.5 mg/l thidiazuron and 1.0 mg/l 6-benzylaminopurine was suitable for callus formation while an improved basal medium i.e., New Winarto–Teixeira-3 supplemented with 0.25 mg/l 2,4-dichlorophenoxy acetic acid, 0.02 mg/l α-naphthalene acetic acid, 1.5 mg/l thidiazuron and 0.75 mg/l 6-benzylaminopurine enhanced callus formation. High shoot regeneration and multiplication was also possible on New Winarto–Teixeira-3. Shoots formed a strong adventitious root system on New Winarto–Teixeira-3 containing 0.2 mg/l α-naphthalene acetic acid and 1.0 mg/l kinetin. Plantlets that varied in size and performance were successfully acclimatized and adapted to ex vitro conditions. Cytological analysis of 180 acclimatized-plantlets ex vitro revealed that 34 were haploid (n = 14–18), 15 aneuploid (n = 20–26), 126 diploid (n = 28–34) and 5 triploid (n = 45–57). The potential use of this protocol for developing half-anther culture of other Anthurium species or cultivars is discussed.

New basal media for half-anther culture of Anthurium andreanum Linden ex André cv. Tropical

https://scielo.conicyt.cl/pdf/ejb/v13n5/a09.pdf

Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter

Anthurium anderanum cv. Terra from family Araceae, is a plant with a high commercial value. Conventional propagation of this plant performed via suckers and seeds planting that nowadays missed its application have been abolished. This study aimed to establish producible protocol for indirect in vitro regeneration of the Terra genotype. For this purpose, the effects of 16 different plant growth regulators treatments on callus induction and regeneration of leaf explants were studied. Callus with the highest fresh and dry weight was produced on modified Murashige and Skoog medium containing 0.1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 1.5 mg/L 6-benzylaminopurine (BAP). For regeneration stage, induced calli were transferred to modified MS medium without plant growth regulators, and after six weeks, shoots formed. For rooting, regenerated shoot were transferred to MS medium without any plant growth regulators. Regenerated plantlets potted on mixture of coco pit and perlite were succesfully acclimated. Key words : Anthurium , micropropagation, growth regulators, regeneration.

/pdf/micropropagation-of-anthurium-andreanum-cv-terra-1afc53ecye.pdf

Micropropagation of Anthurium andreanum cv. Terra

Plant Regeneration by Callus-Mediated Protocorm-Like Body Induction of Anthurium andraeanum Hort.

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Plant Regeneration by Callus-Mediated Protocorm-Like Body Induction of Anthurium andraeanum Hort.