5 Papers
Jing Lu is an academic researcher from Inner Mongolia Agricultural University. The author has contributed to research in topics: Gene knockdown & Cell migration. The author has an hindex of 1, co-authored 5 publications.
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Papers
1'H-Indole-3'-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor's Regulation of Cytoskeletal Contraction.
Lijiao Zhao,Qiuting Shu,Hui Sun,Yunlong Ma,Dandan Kang,Yating Zhao,Jing Lu,Pei Gong,Fan Yang,Fang Wan +9 more
TL;DR: It is found that small molecule 2-(1H-indole-3-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE), an endogenous aryl hydrocarbon receptor (AHR) agonist, can block more than one mode of glioma cell migration, based on cultured cell behavior captured by videos.
Levels of sgRNA as a Major Factor Affecting CRISPRi Knockdown Efficiency in K562 Cells
TL;DR: In this article, K562 cell clones expressing KRAB-dCas9 protein either with the inducible Teton system or with the constitutive SFFV promotor were selected by fluorescence-activated cell sorting (FACS).
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Targeting Aryl Hydrocarbon Receptor with small molecule, 1′H-indole-3′-carbonyl -thiazole-4-carboxylic acid methyl ester blocked human glioma cell invasion via MYH9
li jiao Zhao,Qiu ting Shu,Hui Sun,Yun long Ma,Dan dan Kang,Ya ting Zhao,Jing Lu,Pei Gong,Fan Yang,Fang Wan +9 more
TL;DR: It is found that small molecule 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE), an endogenous AHR ligand, can significantly block glioma cell migration and invasion in vitro, ex vivo and in vivo.
sgRNA level is a major factor affecting CRISPRi knockdown efficiency in K562 cells
TL;DR: In this article, the authors determined the knockdown efficiency and the expression level of the dCas9 protein /sgRNA level by flow cytometry, and the cell clone with the highest KRAB-dCas9 expression level achieved effective CRISPRi knockdown, and is statistically different from other clones.
sgRNA level determines CRISPRi knockdown efficiency in K562 cells
TL;DR: In this paper, the authors determined whether nuclease deactivated Cas9 (dCas9) or sgRNA expression level or both determines the knockdown efficiency of CRISPRi.