Jianghua Li
Jiangnan University
528 Papers
1K Citations
Jianghua Li is an academic researcher from Jiangnan University. The author has contributed to research in topics: Bacillus subtilis & Chemistry. The author has an hindex of 37, co-authored 400 publications.
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Papers
Synergistic regulation of chassis cell growth and screening of promoters, signal peptides and fusion protein linkers for enhanced recombinant protein expression in Bacillus subtilis.
Yao Wu,Long Liu,Jianghua Li,Guocheng Du,Jian Chen,Yanfeng Liu +5 more
Abstract: Growth-advantageous microbial chassis cells are beneficial for shortening fermentation period and boosting biomolecule productivity. This study focused on enhancing recombinant proteins synthesis efficiency in Bacillus subtilis by CRISPRi-mediated metabolism regulation for improved cell growth and screening expression elements. Specifically, by repressing odhA gene expression to reallocate cellular resource and overexpressing atpC, atpD and atpG genes to reprogram energy metabolism, the growth-advantageous chassis cell with high specific growth rate of 0.63 h-1 and biomass yield of 0.41 g DCW/g glucose in minimum medium was developed, representing 61.54 % and 46.43 % increasements compared to B. subtilis 168. Subsequently, using screened optimal P566 promoter and (EAAAK)3 protein linker, secretory bovine alpha-lactalbumin (α-LA) titer reached 1.02 mg/L. Finally, to test protein synthesis capability of cells, intracellular GFP, secretory α-LA and α-amylase were expressed with P566 promoter, representing 43.76 %, 75.49 % and 82.98 % increasements. The growth-advantageous B. subtilis chassis cells exhibit their potential to boost bioproduction productivity.
Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation.
TL;DR: Results revealed that an appropriate sorbitol co-feeding strategy not only decreased the cell mortality in the end of fermentation, but also reduced the proteolytic degradation of PGL.
Comparative genomics and transcriptome analysis of Aspergillus niger and metabolic engineering for citrate production.
TL;DR: Comparative genomics and transcriptome analysis of citrate-producing strains revealed that the transcription levels of 479 genes changed between the cell growth stage and the citrate synthesis stage, and two cytosol ATP-citrate lyases were up-regulated and may coordinate with the alternative oxidases in the alternative respiratory pathway for energy balance.
Synthetic N-terminal coding sequences for fine-tuning gene expression and metabolic engineering in Bacillus subtilis.
TL;DR: Synthetic and native NCSs were used to fine-tune expression of key enzymes, identified via pathway analysis and kinetic modeling, in the biosynthetic pathway of the useful nutraceutical N-acetylneuraminic acid (NeuAc), and a 3.21-fold improvement in NeuAc biosynthesis was observed, indicating that N CSs can provide a synthetic biology toolbox tofine-tunes gene expression for metabolic engineering.
Combination of phenylpyruvic acid (PPA) pathway engineering and molecular engineering of L-amino acid deaminase improves PPA production with an Escherichia coli whole-cell biocatalyst
TL;DR: This study developed a robust whole-cell E. coli biocatalyst for PPA production by integrating metabolic and protein engineering, strategies that may be useful for the construction of other biotransformation bioc atalysts.