DYF371 locus and single-nucleotide polymorphisms (SNPs) defined as DYF371C or DYF371T were simultaneously examined for 142 Chinese unrelated male individuals, including 90 Han population and 52 minorities. In total, 37 SNP-short tandem repeat haplotypes were detected. Nine of these 37 haplotypes were unique (24.32%). The gene diversity and discrimination capacity values are 0.8321 and 0.2606, respectively. Furthermore, only five males (5.55%) in the Han samples were determined that they had one copy of allele with SNP T-type and three copies of alleles with SNP C-types, whereas 14 individuals (26.92%) were observed in minorities' samples. The genotype proportion comprising three distinguishing copies of the allele with SNP C-types in Han sample was greatly lower than that in the sample of minorities. Similar results were shown for allele 11C and 10C. Therefore, the alleles of DYF371 with SNP C-type have the potential to differentiate the Han and non-Han Chinese populations.

/pdf/a-comparative-study-on-the-antimicrobial-activity-of-2rtdnc8mto.pdf

A Comparative Study on the Antimicrobial Activity of Irreversible Hydrocolloid Mixed With Silver Nanoparticles and Chlorhexidine

The Perilla crop is highly regarded in South Korea, both as a health food and traditional food. However, there is still a lack of Perilla SSR primer sets (PSPSs) for studying genetic variation among accessions of cultivated and weedy types of Perilla crop (CWTPC) from South Korea. In this study, 30 PSPSs were newly developed based on transcriptome contigs in P. frutescens, and 17 of these PSPSs were used to study the genetic diversity, phylogenetic relationships and structure population among 90 accessions of the CWTPC collected from South Korea. A total of 100 alleles were detected from selected 17 PSPSs, with an average of 5.9 alleles per locus. The gene diversity (GD) ranged from 0.164 to 0.831, with an average of 0.549. The average GD values from the cultivated var. frutescens, weedy var. frutescens, and weedy var. crispa, were 0.331, 0.588, and 0.389 respectively. In addition, most variance shown by Perilla SSR markers was within a population (73%). An analysis of the population structure and phylogenetic relationships showed that the genetic relationship among accessions of the weedy var. frutescens and weedy var. crispa is closer than that for the accessions of the cultivated var. frutescens. Based on association analysis between 17 PSPSs and three seed traits in 90 Perilla accessions, we detected 11 PSPSs that together were associated with the seed size and seed hardness traits. Therefore, the newly developed PSPSs will be useful for analyzing genetic variation among accessions of the CWTPC, association mapping, and selection of important morphological traits in Perilla crop breeding programs.

/pdf/utilization-of-novel-perilla-ssr-markers-to-assess-the-1juuvx23.pdf

Utilization of Novel Perilla SSR Markers to Assess the Genetic Diversity of Native Perilla Germplasm Accessions Collected from South Korea

Y-chromosome short tandem repeat (Y-STR) and Y-chromosome single nucleotide polymorphism (Y-SNP) are genetic markers on the male Y chromosome for individual identification, forensic applications, and paternal genetic history analysis. In this study we successfully genotyped 38 Y-STR loci and 24 Y-SNP loci of Pudong Han (n = 689) and Chongming Han (n = 530) in Shanghai. The haplotype diversity of the Y filer platinum genotyping system was the highest in the Han population in the Pudong area of Shanghai (0.99996) and Chongming Island (0.99997). The proportion of unique haplotypes was 97.10% (Pudong) and 98.49% (Chongming), respectively. The multidimensional scaling analysis and phylogenetic analysis were performed according to the genetic distance Rst, which was calculated based on the Y-STR gene frequency data. Moreover, we made a comparison on the frequency distribution analysis and principal component analysis of haplogroups in both populations. As a result, Shanghai Pudong Han, Chongming Island Han, and Jiangsu Han were determined to have a strong genetic affinity. The haplogroup distribution characteristics of the Pudong Han and Chongming Han populations were similar to those of the southern Han population. The results of haplotype network analysis showed that Jiangsu Wujiang Han and Jiangsu Changshu Han had more paternal genetic contributions to the formation of Shanghai Pudong Han and Chongming Island Han. Through the joint analysis of SNPs and STRs, this study deeply analyzed the paternal genetic structure of the Pudong Han and Chongming Han populations. The addition of Y-SNP haplogroups to forensic applications can provide information for pedigree investigation.

/pdf/forensic-analysis-and-genetic-structure-construction-of-1sa0sezr.pdf

Forensic Analysis and Genetic Structure Construction of Chinese Chongming Island Han Based on Y Chromosome STRs and SNPs

Forensic characteristic of 19 X-STRs in Chuanqing, Tujia and Yi groups from Guizhou province and their genetic relationships with other reference populations

Abstract   Short tandem repeats (STRs) are the most common genetic markers in forensic and human population genetics due to their high polymorphism, rapid detection, and reliable genotyping. To adapt the rapid growth of forensic DNA database and solve problems in disputed cases, a panel of 23 autosomal STR loci with high discriminating ability was constructed recently. The Tai-Kadai-speaking Gelao is the most ancient indigenous minority in Guizhou province, however, the forensic efficiency and population genetic structure remain poorly explored. Here, 490 Guizhou Gelao individuals from Southwest China were genotyped with the panel of 23 STRs using the Huaxia Platinum Kit. A total of 265 alleles were screened. The combined discrimination power and the combined probability of paternity were 0.9999 and 0.9999, respectively. This indicated the 23 loci had higher discrimination power in Guizhou Gelao and could be applied to forensic practice. Comprehensive population structures with reference populations from China and abroad using the neighbour-joining phylogenetic tree (N-J tree), multidimensional scaling, principal component analysis and heatmap demonstrated that Guizhou Gelao was genetically closer to Guizhou Han than other populations. Moreover, our results showed that a complex phylogenetic model was influenced by ethnic, geographic, and linguistic factors. Key points The first batch of genetic data for 23 autosomal STRs in 490 Geolao individuals from Guizhou was provided. The 23 STR panel can afford high genetic polymorphisms and discrimination power and can be efficiently applied to forensic practice in Guizhou Gelao population. A complex phylogenetic model influenced by ethnic, geographic, and linguistic factors was uncovered.

Forensic efficiency and population genetic construction of Guizhou Gelao minority from Southwest China revealed by a panel of 23 autosomal STR loci

Genetic polymorphisms and haplotypic structure analysis of the Guizhou Gelao ethnic group based on 35 Y-STR loci.

The invention discloses a method for identifying a tissue source of body fluid. The method comprises the following steps: detecting the expression quantities of six miRNAs, namely miRNA 205, miRNA 451, miRNA 144, miRNA 214, miRNA 888 and miRNA 891 in the body fluid and then calculating a probability value of the to-be-detected body fluid from the tissue source by adopting a Softmax regression model and a classified one-hot form, wherein the maximum probability value is the tissue source of the body fluid. By adopting the method disclosed by the invention, the tissue source of the body fluid can be effectively identified, so that the step of identifying the tissue source of the body fluid or body fluid spots at the scene of criminal investigation is greatly simplified, and an accurate scientific basis can be provided for case nature identification, suspect identification, conviction and sentence, and the like.

Method for identifying tissue source of body fluid and special miRNA combination thereof

The invention discloses a multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and a primer combination used by the multiplex amplification system. The primer combination is composed of 12 DNA molecules shown in sequences 1 to 12 in a sequence table. By adopting the primer combination to construct the multiplex amplification system, whether body fluid to be detected is non-blood, menstrual blood or peripheral blood can be identified, and the accuracy is high. The multiplex amplification system provides an accurate scientific basis for defining case properties, determining crime sentences and the like, and has important application value.

Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system

The invention discloses an miRNAs multiplex amplification system for identifying tissue origin of human body fluid and a special primer combination thereof. The primer combination comprises a first primer combination and a second primer combination, wherein the first primer combination consists of 16 primers, and the nucleotide sequences are sequentially shown as sequence 21 to sequence 28 and sequence 31 to sequence 38; the second primer combination consists of 8 primers, and the nucleotide sequences are sequentially shown as a sequence 11, a sequence 12, a sequence 13, a sequence 14, a sequence 16, a sequence 17, a sequence 18 or a sequence 19. When the miRNAs multiplex amplification system provided by the invention is used, the tissue origin of human body fluid can be effectively identified; the steps for identifying the tissue origin of forensic field body fluid or spots are greatly simplified; an accurate scientific basis can be provided for determining the case character, determining suspects, realizing the conviction and sentencing and the like. Important application values are realized.

MiRNAs multiplex amplification system for identifying tissue origin of human body fluid and special primer combination thereof

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Genetic polymorphisms and haplotypic structure analysis of the Guizhou Gelao ethnic group based on 35 Y-STR loci.

Method for identifying tissue source of body fluid and special miRNA combination thereof

Multiplex amplification system for identifying whether body fluid to be detected is non-blood, menstrual blood or peripheral blood and primer combination used by multiplex amplification system

MiRNAs multiplex amplification system for identifying tissue origin of human body fluid and special primer combination thereof