Janet Estee Kacharmina
University of Pennsylvania
11 Papers
378 Citations
Janet Estee Kacharmina is an academic researcher from University of Pennsylvania. The author has contributed to research in topics: Population & Gene expression profiling. The author has an hindex of 8, co-authored 11 publications.
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Papers
Local translation of classes of mRNAs that are targeted to neuronal dendrites.
TL;DR: Conclusive evidence that these mRNAs can be translated is reviewed, and identification of the endogenous sites of translation in living dendrites is presented, highlighting the complexity of dendritic molecular biology and the exquisitely selective and sensitive modulatory role played by the dendrite in facilitating intracellular and intercellular communication.
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Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites
TL;DR: It is shown that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation, and confocal imaging of N-terminally labeled GLUR2 reveals that the newly synthesized Glu R2 protein can integrate into the dendritic membrane with the N terminus externally localized.
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Analysis of subcellularly localized mRNAs using in situ hybridization, mRNA amplification, and expression profiling.
TL;DR: In this chapter the methods for analysis of RNAs that are localized to dendrites are reviewed and a partial list of dendritically localized RNAs is presented, which may be useful in identifying RNA regulatory regions that are responsible for specifying rate of RNA transport and the dendritic sites at which targeted RNAs dock so that they can be translated.
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Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution
TL;DR: With the linear amplification ability of T7 RNA polymerase, IDAT represents a significant improvement over immuno-PCR in terms of sensitivity and has the potential to provide a robotic platform for proteomics.
109
mRna expression analysis of tissue sections and single cells.
James Eberwine,Janet Estee Kacharmina,Christine Andrews,Kevin Miyashiro,Tracy K. McIntosh,Kevin G. Becker,Tanya Barrett,Dave Hinkle,Gersham W. Dent,Paolo G. Marciano +9 more
TL;DR: Thirty years of literature have shown that changes in mRNA abundance provide insight into cellular functioning and how effective particular drugs are in eliciting cellular responses, in monitoring behavioral responses, and in several other studies.
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