Jane Theaker
AstraZeneca
8 Papers
306 Citations
Jane Theaker is an academic researcher from AstraZeneca. The author has contributed to research in topics: Primer extension & Nucleic acid. The author has an hindex of 7, co-authored 8 publications.
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Papers
Detection of PCR products using self-probing amplicons and fluorescence.
TL;DR: This work describes a new technology that is simple to use, gives highly specific information, and avoids the major difficulties of the alternative methods of molecular diagnostics.
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Patent
Methods for detecting target nucleic acid sequences
David Mark Whitcombe,Jane Theaker,Neil James Gibson,Stephen Little +3 more
- 25 Nov 1998
TL;DR: In this article, a method for the detection of a target nucleic acid was proposed, which consisted of contacting template nucleic acids from a sample with a signalling system and a tailed nuclei acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerization thereof.
144
A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping
David Mark Whitcombe,Jannine Brownie,Helen L. Gillard,Doug McKechnie,Jane Theaker,Clive R. Newton,Stephen Little +6 more
TL;DR: Three-STAR is a universal system that can either use a single probe for the detection of any one target DNA sequence or a single pair of probes for genotyping any bi-allelic polymorphism and is particularly useful for the single-tube genotype analysis of a variety of human DNA polymorphisms and mutations.
Patent
Methods and primers for detecting target nucleic acid sequences
David Mark Whitcombe,Jane Theaker,Neil James Gibson,Stephen Little +3 more
- 08 Jun 2004
TL;DR: In this article, a method for the detection of a target nucleic acid was proposed, which consisted of contacting template nucleic acids from a sample with a signalling system and a tailed nuclei acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerisation thereof.
29
Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America
Thomas Edwards,Leticia del Carmen Castillo Signor,Christopher T Williams,Clément Larcher,Mauricio Espinel,Jane Theaker,Evelin Donis,Luis E. Cuevas,Emily R. Adams +8 more
TL;DR: A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary strains and found to have a sensitivity and specificity of 98.4% and 100% in 90 samples retrospectively collected in Guatemala.
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