Jan E. Blanchard
McMaster University
26 Papers
93 Citations
Jan E. Blanchard is an academic researcher from McMaster University. The author has contributed to research in topics: Glucocerebrosidase & Myeloid leukemia. The author has an hindex of 12, co-authored 26 publications.
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Papers
Identification of Pharmacological Chaperones for Gaucher Disease and Characterization of Their Effects on β‐Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry
Michael B. Tropak,G. Kornhaber,Brigitte Rigat,Gustavo Maegawa,Justin D. Buttner,Jan E. Blanchard,Cecilia Murphy,Steven J. Tuske,Stephen J. Coales,Yoshitomo Hamuro,Eric D. Brown,Don J. Mahuran +11 more
TL;DR: Changes in protein dynamics, monitored by hydrogen/deuterium‐exchange mass spectrometry, identified a domain III active‐site loop as being significantly stabilized upon binding of isofagomine or either of these two new compounds; this suggests a common mechanism for PC enhancement of intracellular transport.
High-Throughput Screening Identifies Novel Inhibitors of the Acetyltransferase Activity of Escherichia coli GlmU
TL;DR: An absorbance-based assay is developed to screen diverse chemical libraries in high throughput for inhibitors to the acetyltransferase reaction of Escherichia coli GlmU and outlines the utility of catalytic variants in targeting specific activities of bifunctional enzymes in high-throughput screens.
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Inhibitors of bacterial cystathionine beta-lyase: leads for new antimicrobial agents and probes of enzyme structure and function.
Linda Ejim,Jan E. Blanchard,Kalinka Koteva,Rachael Sumerfield,Nadine H. Elowe,Jonathan D. Chechetto,Eric D. Brown,Murray S. Junop,Gerard D. Wright +8 more
TL;DR: These studies provide the first lead molecules for antimicrobial agents that target cystathionine beta-lyase in methionine biosynthesis, and determine the crystal structure of one of the slow-binding inhibitors in complex with CBL and used it as a guide in the synthesis of a small, focused library of analogues, some of which had improved enzyme inhibition properties.
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Experimental screening of dihydrofolate reductase yields a "test set" of 50,000 small molecules for a computational data-mining and docking competition.
TL;DR: High-throughput screening generates an abundance of data that are a valuable resource to be mined and computational chemists and data analysts were challenged to predict the activity of compounds in this second “test set.”
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A Pipeline for Screening Small Molecules with Growth Inhibitory Activity against Burkholderia cenocepacia
Carrie Selin,Maria S. Stietz,Jan E. Blanchard,Sebastian S. Gehrke,Sylvain Bernard,Dennis G. Hall,Eric D. Brown,Silvia T. Cardona +7 more
TL;DR: A whole-cell based assay is used to screen a diverse collection of small molecules for growth inhibitors of a relevant strain of Bcc, highlighting the utility of this experimental method to quickly identify bioactives as a starting point of antibacterial drug discovery.