James Li
Sangamo BioSciences
4 Papers
2 Citations
James Li is an academic researcher from Sangamo BioSciences. The author has contributed to research in topics: DNA repair & Recombinase. The author has an hindex of 2, co-authored 3 publications.
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Papers
DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.
Deniz Simsek,Erika Brunet,Erika Brunet,Sunnie Yan-Wai Wong,Sachin Katyal,Yankun Gao,Peter J. McKinnon,Jacqueline F. Lou,Lei Zhang,James Li,Edward J. Rebar,Philip D. Gregory,Michael C. Holmes,Maria Jasin,Maria Jasin +14 more
TL;DR: Investigation of the DNA ligase requirement of chromosomal translocation formation in mouse cells finds the existence of two alt-NHEJ pathways, one that is biased toward microhomology use and requires Lig3 and a back-up pathway which does not depend on microHomology and utilizes Lig1.
Phenotypic Correction of a Mouse Model of Hemophilia B by In Vivo Genetic Correction of the F9 Gene
Hojun Li,Virginia Haurigot,Yannick Doyon,James Li,Anand S. Bhagwat,Sunnie Wong,Xavier M. Anguela,Rajiv Sharma,Lacramioara Ivanciu,Samuel L. Murphy,Shangzhen Zhou,David Paschon,Edward J. Rebar,Philip D. Gregory,Michael C. Holmes,Katherine A. High +15 more
TL;DR: ZFN-mediated therapeutic gene targeting of a mutated F9 gene in vivo is shown, resulting in phenotypic correction of a mouse model of hemophilia B (HB), establishing a novel paradigm for in vivo gene correction as a method for treating inherited hematologic diseases.
2
Adaptation of a Simulation Model and Checklist to Assess Pediatric Emergency Care Performance by Prehospital Teams
Tehnaz P. Boyle,Julianne Dugas,James Li,Stephanie Stapleton,Ron Medzon,Barbara Walsh,Pamela Corey,Leonard Shubitowski,John Robert Horne,Richard O'Connell,Graham Williams,Kerrie P. Nelson,Vinay M. Nadkarni,Carlos A. Camargo,James A. Feldman +14 more
TL;DR: The modified checklist has very good agreement for assessing composite prehospital team performance and can be used to test effects of patient safety interventions.
1
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology
Salvatore J. Orlando,Yolanda Santiago,Russell Dekelver,Yevgeniy Freyvert,Elizabeth A. Boydston,Erica A. Moehle,Vivian M. Choi,Sunita Gopalan,Jacqueline F. Lou,James Li,Jeffrey C. Miller,Michael C. Holmes,Philip D. Gregory,Fyodor D. Urnov,Gregory J. Cost +14 more
TL;DR: It is demonstrated that easily-generated linear donors with extremely short homology regions drive transgene integration into 5–10% of chromosomes and that oligonucleotide donors with single-stranded 5′ overhangs complementary to those made by ZFNs are efficiently ligated in vivo to the DSB.