James J. Burchall
Research Triangle Park
16 Papers
338 Citations
James J. Burchall is an academic researcher from Research Triangle Park. The author has contributed to research in topics: Dihydrofolate reductase & Trimethoprim. The author has an hindex of 12, co-authored 16 publications.
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Papers
Purification and properties of Escherichia coli dihydrofolate reductase.
TL;DR: Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography.
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Mechanism of Action of Trimethoprim-Sulfamethoxazole—II
TL;DR: It is concluded that the enzymatic locus of action of the compound is dihydrofolate reductase, and the selective action of TMP is based on its ability to distinguish structural differences in reductases isolated from various organisms.
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ADP-ribosyltransferase activity of pertussis toxin and immunomodulation by Bordetella pertussis
W. J. Black,J. J. Munoz,M. G. Peacock,PA Schad,JL Cowell,James J. Burchall,Mae Lim,Andrew D. Kent,Lawrence Steinman,Stanley Falkow +9 more
TL;DR: It is suggested that the ADP-ribosyltransferase activity is necessary for both pathogenicity and optimum immunoprotection in pertussis vaccine.
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Crystal structure of a novel trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67.
TL;DR: A hypothetical model is proposed for the R67 DHFR-NADPH-folate ternary complex that is consistent with both the known reaction stereoselectivity and the weak binding of 2,4-diamino inhibitors to the plasmid-specified reductase.
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Identification of Harper-Cawston Factor as Thymidine Phosphorylase and Removal from Media of Substances Interfering with Susceptibility Testing to Sulfonamides and Diaminopyrimidines
TL;DR: The identification of HCF as Thymidine phosphorylase is consistent with the earlier findings of Koch and Burchall (1971) that most of the interfering effects of rich media could be accounted for by their thymidine contents, and that thymazine is much more active than is thymine.
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