James Gowdy

TL;DR: Data on the rapid reduction of haem proteins in the X-ray beam at synchrotron sources and the use of single-crystal spectroscopy to detect these changes and their implication for diffraction data collection from oxidized species are presented.

TL;DR: It is demonstrated that high-resolution TROSY NMR spectra can be achieved with large complex α-helical membrane proteins without the use of elevated temperatures and is a prerequisite to applying further labelling strategies and NMR experiments for measurement of dynamics, structure elucidation and use of the spectra to screen ligand binding.

TL;DR: The utility of constant force to enhance the sampling efficiency and obtain experimentally measurable quantities on a series of short peptides, including charge-rich sequences that are known to be highly helical but whose properties are distinct from those of helical peptides undergoing helix-coil transitions.

TL;DR: The structure of PanZ in complex with PanD is reported, which reveals the structural basis for the CoA dependence and shows that PanZ acts as a CoA-dependent inhibitor of ADC catalysis, which represents a previously unobserved mode of metabolic regulation whereby a cofactor-utilizing protein negatively regulates the biosynthesis of the same cofactor.