James A. Kissel
University of Cincinnati Academic Health Center
3 Papers
17 Citations
James A. Kissel is an academic researcher from University of Cincinnati Academic Health Center. The author has contributed to research in topics: Complementary DNA & Biology. The author has an hindex of 3, co-authored 3 publications.
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Papers
Molecular cloning and expression of cDNA for rat pancreatic cholesterol esterase.
TL;DR: Analysis of the cholesterol esterase structure revealed a repetitive sequence enriched with Pro, Asp, Glu, Ser, and Thr residues at the C-terminal end of the protein, which is reminiscent of the PEST-rich sequences in short-lived proteins, suggesting that cholesterol Esterase may have a short half-life in vivo.
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Purification of pancreatic cholesterol esterase expressed in recombinant baculovirus-infected Sf9 cells.
TL;DR: It is demonstrated that Baculovirus-infected Sf9 cells can be used for high-level expression of pancreatic cholesterol esterase and the recombinant enzyme will be useful for further characterization of this protein.
18
Sequence identity between human pancreatic cholesterol esterase and bile salt-stimulated milk lipase.
David Y. Hui,James A. Kissel +1 more
TL;DR: Analysis of the sequence for the human cholesterol esterase/milk lipase revealed similarities to other serine esterases in three distinct regions of the protein, which may represent the active site triads of these proteins.