Jacob S. Lewis
Illawarra Health & Medical Research Institute
26 Papers
6 Citations
Jacob S. Lewis is an academic researcher from Illawarra Health & Medical Research Institute. The author has contributed to research in topics: DNA replication & Replisome. The author has an hindex of 10, co-authored 22 publications. Previous affiliations of Jacob S. Lewis include Francis Crick Institute & University of Wollongong.
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Papers
Single-molecule visualization of fast polymerase turnover in the bacterial replisome
Jacob S. Lewis,Jacob S. Lewis,Lisanne M. Spenkelink,Lisanne M. Spenkelink,Lisanne M. Spenkelink,Slobodan Jergic,Slobodan Jergic,Elizabeth A. Wood,Enrico Monachino,Enrico Monachino,Enrico Monachino,Nicholas P Horan,Nicholas P Horan,Karl E. Duderstadt,Karl E. Duderstadt,Karl E. Duderstadt,Michael M. Cox,Andrew Robinson,Andrew Robinson,Nicholas E. Dixon,Nicholas E. Dixon,Antoine M. van Oijen,Antoine M. van Oijen +22 more
TL;DR: In vitro single-molecule assays with fluorescently labeled polymerases demonstrate that the Pol III* complex, is rapidly exchanged during processive DNA replication, suggesting a concentration-dependent exchange mechanism providing a balance between stability and plasticity, facilitating replacement of replisomal components dependent on their availability in the environment.
Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication
Jacob S. Lewis,Jacob S. Lewis,Lisanne M. Spenkelink,Lisanne M. Spenkelink,Grant D. Schauer,Olga Yurieva,Olga Yurieva,Stefan H. Mueller,Stefan H. Mueller,Varsha Natarajan,Varsha Natarajan,Gurleen Kaur,Gurleen Kaur,Claire Maher,Claire Maher,Callum Kay,Callum Kay,Mike O'Donnell,Mike O'Donnell,Antoine M. van Oijen,Antoine M. van Oijen +20 more
TL;DR: It is shown that Pol α-primase and the lagging-strand Pol δ can be re-used within the replisome to support the synthesis of large numbers of Okazaki fragments, which might allow it to deal with barriers and resource challenges during replication of large genomes.
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Mechanism of replication origin melting nucleated by CMG helicase assembly
Jacob S. Lewis,Marta Gross,Joana Sousa,Sarah S. Henrikus,Julia F. Greiwe,Andrea Nans,John F.X. Diffley,Alessandro Costa +7 more
TL;DR: In this article , the authors used cryo-electron microscopy to image ATP-dependent CMG assembly on a chromatinized origin, reconstituted in vitro with purified yeast proteins.
Spatial and temporal organization of RecA in the Escherichia coli DNA-damage response.
Harshad Ghodke,Harshad Ghodke,Bishnu P. Paudel,Bishnu P. Paudel,Jacob S. Lewis,Jacob S. Lewis,Slobodan Jergic,Slobodan Jergic,Kamya Gopal,Zachary J Romero,Elizabeth A. Wood,Roger Woodgate,Michael M. Cox,Antoine M. van Oijen +13 more
TL;DR: A monomeric C-terminal fragment of the λ repressor is developed as a novel fluorescent probe that specifically interacts with RecA filaments on single-stranded DNA (RecA*), demonstrating that RecA is largely sequestered in storage structures during normal metabolism.
Recycling of single-stranded DNA-binding protein by the bacterial replisome.
Lisanne M. Spenkelink,Lisanne M. Spenkelink,Lisanne M. Spenkelink,Jacob S. Lewis,Jacob S. Lewis,Slobodan Jergic,Slobodan Jergic,Zhi-Qiang Xu,Zhi-Qiang Xu,Andrew Robinson,Andrew Robinson,Nicholas E. Dixon,Nicholas E. Dixon,Antoine M. van Oijen,Antoine M. van Oijen +14 more
TL;DR: This work visualises how an in vitro reconstituted E. coli replisome recruits SSB by relying on two different molecular mechanisms, and shows that both internal transfer and external exchange mechanisms are physiologically relevant.
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