Jaclyn M. Nascimento
University of California, San Diego
16 Papers
128 Citations
Jaclyn M. Nascimento is an academic researcher from University of California, San Diego. The author has contributed to research in topics: Sperm & Sperm motility. The author has an hindex of 10, co-authored 16 publications. Previous affiliations of Jaclyn M. Nascimento include University of California.
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Papers
Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real‐time automated tracking and trapping
Jaclyn M. Nascimento,Linda Z. Shi,James Tam,Charlie Chandsawangbhuwana,Barbara S. Durrant,Elliot L. Botvinick,Michael W. Berns,Michael W. Berns +7 more
TL;DR: The results indicate that oxidative phosphorylation does contribute some ATP for human sperm motility, but not enough to sustain high motility.
Computer-based tracking of single sperm
TL;DR: A robust single sperm tracking algorithm (SSTA) that can be used in laser optical trapping and sperm motility studies and is validated through examples and comparisons to commercially available computer-aided sperm tracking systems.
Real-time automated tracking and trapping system for sperm.
Linda Z. Shi,Jaclyn M. Nascimento,Charlie Chandsawangbhuwana,Michael W. Berns,Michael W. Berns,Elliot L. Botvinick +5 more
TL;DR: A microscope system for real‐time single sperm tracking with an automated laser tweezers escape power assay and the efficacy of the “track and trap” algorithm is validated through examples and comparisons with the manually collected data.
An automatic system to study sperm motility and energetics
Linda Z. Shi,Jaclyn M. Nascimento,Charlie Chandsawangbhuwana,Elliot L. Botvinick,Elliot L. Botvinick,Michael W. Berns,Michael W. Berns,Michael W. Berns +7 more
TL;DR: An integrated robotic laser and microscope system has been developed to automatically analyze individual sperm motility and energetics that has not only increased experimental throughput by an order of magnitude but also has allowed us to monitor sperm ener getics prior to and after exposure to the laser trap.
Use of laser tweezers to analyze sperm motility and mitochondrial membrane potential.
Jaclyn M. Nascimento,Linda Z. Shi,Charlie Chandsawangbhuwana,James Tam,Barbara S. Durrant,Elliot L. Botvinick,Michael W. Berns +6 more
TL;DR: The system's capabilities are demonstrated by measuring VCL, Pesc, and MP simultaneously for individual sperm by combining laser tweezers with computer tracking software and robotics to quantitatively assess sperm quality and viability.