The year 2006 marks the 50th anniversary of the discovery of cellulose acetate (CA) electrophoresis by Joachim Kohn, a pathologist at Queen Mary's Hospital in Roehampton, London. During a career in pathology that began in 1950 and spanned 37 years, Kohn published more than 50 papers in clinical laboratory medicine. He was the first to report the use of CA microbiology filters as solid supports for zone electrophoresis and the separation of hemoglobin phenotypes on CA membranes. Kohn also invented a new electrophoresis chamber and an 8-position stamp applicator especially for use with CA membranes. Beginning in 1957, Kohn pioneered the development of CA techniques for immunoelectrophoresis, counter immunoelectrophoresis, radial immunodiffusion, protein blotting, and immunofixation. He also designed a transport dressing for burn patients and was the first person to describe the use of an enzyme-based dipstick for measuring fingerstick blood glucose concentrations. This short review highlights Kohn's discovery of CA electrophoresis and his contributions to the development of this procedure.

/pdf/joachim-kohn-1912-1987-and-the-origin-of-cellulose-acetate-5evbnt54o6.pdf

Joachim Kohn (1912–1987) and the Origin of Cellulose Acetate Electrophoresis

Immunofixation electrophoresis with use of Beckman Paragon SPE gels.

The review presents a chronological development of procedures for determining the concentrations of total protein and albumin; total concentration of globulins; concentrations of subfractions, classes, and subclasses of globulins; and total concentrations of globulin subclasses in human blood. A brief overview of these procedures and the results of their comparative tests in medical examination of patients are given. The considered procedures are systematized; their advantages and disadvantages are discussed. It is noted that procedures for measuring the concentration of total protein, albumin, and globulin classes in plasma, serum, and whole blood are widely used in modern clinical diagnostic laboratories when performing routine assays. The concentrations of globulin subclasses and total concentrations of globulin subclasses in blood are mainly determined in biomedical research. Procedures for measuring the concentration of total globulins and globulin subfractions in plasma and serum are of historical importance and now are barely applied in clinical and diagnostic laboratories.

Determination of proteins in blood. Part 3: Systematization of methods for the determination of protein compounds in blood

CHAPTER 6 – Immunofixation Technique

The review presents the chronological development of procedures for determining the concentrations of total protein and albumin; total concentration of globulins; concentrations of subfractions, classes, and subclasses of globulins; and total concentrations of globulin subclasses in human blood A brief overview of these procedures and the results of their comparative tests in medical examination of patients are given The considered procedures are systematized; their advantages and disadvantages are discussed It is noted that procedures for measuring the concentration of total protein, albumin, and globulin classes in plasma, serum, and whole blood are widely used in modern clinical diagnostic laboratories when performing routine assays The concentrations of globulin subclasses and total concentrations of globulin subclasses in blood are mainly determined in biomedical research Procedures for measuring the concentration of total globulins and globulin subfractions in plasma and serum are of historical importance and now are barely applied in clinical and diagnostic laboratories

Determination of proteins in blood. Part 2: Determination of globulins

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