Fertile egg windowing to access bird embryo is a useful technique in transgenic birds,conserving bird genetic resources with pluripotent stem cells as well as experimental embryo model fields.We invented a technique using newly laid fertile eggs,which the cover cut from the eggshell could recover automatically on the window.Based on this technique several factors affecting the hatchability of the windowed eggs(HWE) were investigated,such as different seal method,different windowing spot on the eggshell and adding liquid to drive air bubbles within the windowed eggs.It was indicated that the seal methods here did not remarkably affect the HWE and the highest results got up to 65 %.It seemed as if that adding liquid to move the air bubbles did not help to increase the HWE,and the volume of the bladder has also no visible effect on the HWE;The HWE of the method,which windowed on the blunt end of the eggs(access to the embryo by stinging through the inner membrane) was much higher than that of windowed on equatorial plane,and the rate of both methods to find the embryos was 90 %;The efficiency was 30 eggs for one person one hour using the above methods to window,find embryo and recover the eggs.The technique reported here was very efficiency and practical,and it would do great helps to the research on transgenic poultry as well as relative fields.

A Study on Fertile Egg Windowing and Its Effect on Embryo Development

In order to find out the main factors influenced the hatchability and improve the hatchability of the windowed chicken eggs at stage X, several experiments were made on the basis of the former patent of eggshell windowing methods on equatorial plane, such as cutting and sealing techniques, air cell recovering, laying position immediately after sealing, as well as the injection volumes into the subgerminal cavity of the blastoderm. The result showed that:1) the best sealing material combination was straw powder (SP) and instant glue (IG); 2) there was a highly positive correlation between air cell rate and hatchability; 3) the highest hatchability increased to 71.6% when the eggs were windowed and sealed with IG dropped firstly and then SP sprinkled, finally lay down with the blunt end upward immediately after being sealed; 4) the hatchability was significantly reduced as injection volume (DMEM) was increased (p<0.05 or p<0.01) from 1 µL to 10 1 µL, and the group of injecting 1 µL was the highest (48.4 %). The hatchability and efficiency with such method of windowing, injecting, and sealing was the highest at the present time (more than 30 eggs per hour per person), and it might be broadly used in the fields of avian transgenesis, genetic resources preservation, and embryonic development model of human medicine.

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Study on the Efficient Methods for Chicken Eggshell windowing and the Factors Affecting the Hatchability

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Study on the Efficient Methods for Chicken Eggshell windowing and the Factors Affecting the Hatchability