In this study a new approach is presented for on-line radiometric detection in reversed phase LC of medium to low polarity compounds labelled with14C.

Low-level radiometric detection in LC using solvent segmentation and an effluent storage loop

Publisher Summary This chapter discusses radiochromatographic techniques. The chromatography of radioactive substances is encountered in many fields, but especially in biochemistry and the biological sciences. In radiochemistry, chromatography is used for the isolation and analysis of the products of nuclear and chemical reactions. The chromatographic technique as such, involving sample introduction, columns, sorbents, developers, and a pumping system, is used for radioactive substances and does not differ in any essential respect from the techniques used for stable nuclides. The possibility that radioactive reaction products such as tritiated water from tritiated compounds and 14CO2 from 14C-labelled compounds may be generated should also be considered and atmospheric contamination avoided. Radiation detectors used during or after liquid column chromatography differ according to the type of involved radiation, its energy, and sometimes its level. The usual method for detecting γ-radiation involves the use of an aluminum-covered crystal of thallium-activated sodium iodide coupled to a photomultiplier.

Chapter 16 Radiochromatographic techniques

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Chapter 16 Radiochromatographic techniques