Isabelle Pot
University of British Columbia
7 Papers
31 Citations
Isabelle Pot is an academic researcher from University of British Columbia. The author has contributed to research in topics: Kinetochore & Centromere. The author has an hindex of 7, co-authored 7 publications.
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Papers
Identification of Protein Complexes Required for Efficient Sister Chromatid Cohesion
Melanie L. Mayer,Isabelle Pot,Michael Chang,Hong Xu,Victoria Aneliunas,Teresa Kwok,Rick Newitt,Ruedi Aebersold,Charles Boone,Grant W. Brown,Philip Hieter +10 more
TL;DR: The data indicate that synthetic genetic array analysis coupled with specific secondary screens can effectively identify protein complexes functionally related to a reference gene and find that genes involved in mitotic spindle integrity and positioning have a previously unrecognized role in sister chromatid cohesion.
Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase
Manish D. Joshi,Gary Sidhu,Isabelle Pot,Gary D. Brayer,Stephen G. Withers,Lawrence P. McIntosh +5 more
TL;DR: It is proposed that Asp35 and Glu172 function together as the general acid/base catalyst, and that N35D BCX exhibits a "reverse protonation" mechanism in which it is catalytically active when Asp 35, with the lower pKa, is protonated, while Glu78, withThe higher p Ka, is deprotonated.
223
Systematic yeast synthetic lethal and synthetic dosage lethal screens identify genes required for chromosome segregation
Vivien Measday,Kristin Baetz,Julie Guzzo,Karen Yuen,Teresa Kwok,Bilal N. Sheikh,Huiming Ding,Ryo Ueta,Trinh Hoac,Benjamin Cheng,Isabelle Pot,Amy Hin Yan Tong,Yuko Yamaguchi-Iwai,Charles Boone,Phil Hieter,Brenda J. Andrews +15 more
TL;DR: This study shows that systematic genetic screens are a powerful means to discover roles for uncharacterized genes and genes with alternative functions in chromosome maintenance that may not be discovered by using proteomics approaches.
150
Spindle checkpoint maintenance requires Ame1 and Okp1.
Isabelle Pot,James Knockleby,Victoria Aneliunas,Thao T. T. Nguyen,Sonia Ah-Kye,Gregory Liszt,Michael Snyder,Philip Hieter,Jackie Vogel +8 more
TL;DR: It is proposed that Ame1 and Okp1 are required for a sustained checkpoint arrest in the presence of mis-segregated chromosomes and the results suggest that checkpoint response might be controlled not only at the level of activation but also via signals that ensure maintenance of the response.
34
Chl4p and iml3p are two new members of the budding yeast outer kinetochore.
Isabelle Pot,Vivien Measday,Brian E. Snydsman,Gerard Cagney,Stanley Fields,Trisha N. Davis,Eric G D Muller,Philip Hieter +7 more
TL;DR: It is established that yeast Chl4p and Iml3p are outer kinetochore proteins that localize to the kinetchore in a Ctf19p-dependent manner, and these physical interaction dependencies provide insights into the molecular architecture and centromere DNA loading requirements of the outer kinETochore complex.