Ilya A. Shestopalov
Stanford University
12 Papers
17 Citations
Ilya A. Shestopalov is an academic researcher from Stanford University. The author has contributed to research in topics: Morpholino & Oligonucleotide. The author has an hindex of 9, co-authored 12 publications.
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Papers
Light-controlled gene silencing in zebrafish embryos
TL;DR: The synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.
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Versatile synthesis and rational design of caged morpholinos.
Xiaohu Ouyang,Ilya A. Shestopalov,Surajit Sinha,Genhua Zheng,Cameron L W Pitt,Wen Hong Li,Andrew Olson,James K. Chen +7 more
TL;DR: Optimize methods for the design and synthesis of hairpin cMOs incorporating a dimethoxynitrobenzyl (DMNB)-based bifunctional linker that permits cMO assembly in only three steps from commercially available reagents establish the generality of cMO technologies and will facilitate the application of these chemical probes in vivo for functional genomic studies.
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Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function
TL;DR: Using morpholino-based antisense reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined and photocontrol of gene expression in zebrafish embryos and larvae is enabled.
Post-transcriptional mechanisms contribute to Etv2 repression during vascular development.
John C. Moore,Sarah Sheppard-Tindell,Ilya A. Shestopalov,Sayumi Yamazoe,James K. Chen,Nathan D. Lawson +5 more
TL;DR: The results suggest that etv2 acts during early development to specify endothelial lineages and is then down-regulated, in part through post-transcriptional repression by microRNAs, to allow normal vascular development.
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Oligonucleotide-Based Tools for Studying Zebrafish Development
TL;DR: The capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression are surveyed and recent strategies for achieving spatiotemporal control of oligon nucleotide function are examined.
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