12 Papers
12 Citations
Igor Orlov is an academic researcher from French Institute of Health and Medical Research. The author has contributed to research in topics: Super-resolution microscopy & Chemistry. The author has an hindex of 8, co-authored 10 publications. Previous affiliations of Igor Orlov include Centre national de la recherche scientifique.
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Papers
ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy.
TL;DR: ClusterViSu is described, a method for image reconstruction, visualization and quantification of labelled protein clusters, based on Voronoi tessellation of the individual fluorescence events, for super-resolution microscopy data segmentation.
Protein Delivery System Containing a Nickel‐Immobilized Polymer for Multimerization of Affinity‐Purified His‐Tagged Proteins Enhances Cytosolic Transfer
Viktoriia Y. Postupalenko,Dominique Desplancq,Igor Orlov,Youri Arntz,Danièle Spehner,Yves Mély,Bruno P. Klaholz,Patrick Schultz,Etienne Weiss,Guy Zuber +9 more
TL;DR: Transduction of the protease caspase 3 induced apoptosis in two cancer cell lines, demonstrating that this new protein delivery method could be used to interfere with cellular functions.
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The integrative role of cryo electron microscopy in molecular and cellular structural biology.
Igor Orlov,Alexander G. Myasnikov,Leonid Andronov,S. Kundhavai Natchiar,Heena Khatter,Brice Beinsteiner,Jean-François Ménétret,Isabelle Hazemann,Kareem Mohideen,Karima Tazibt,Rachel Tabaroni,Hanna Kratzat,Nadia Djabeur,Tatiana Bruxelles,Finaritra Raivoniaina,Lorenza di Pompeo,Morgan Torchy,Isabelle M. L. Billas,Alexandre Urzhumtsev,Bruno P. Klaholz +19 more
TL;DR: Multi‐scale and multi‐resolution approaches allow integrating molecular and cellular levels when applied to purified or in situ macromolecular complexes, thus illustrating the trend of the field towards cellular structural biology.
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Cryo-FIB-SEM as a promising tool for localizing proteins in 3D
Danièle Spehner,Anna M. Steyer,Luca Bertinetti,Igor Orlov,Lucas Benoit,Karin Pernet-Gallay,Andreas Schertel,Patrick Schultz +7 more
TL;DR: Vitrified human HeLa cells are vitrified and directly imaged by cryo-FIB-SEM with the secondary electron InLens detector at cryogenic temperature and without any staining and gold-conjugated antibodies directed against RNA polymerase II could be observed in the endo-lysosomal pathway.
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3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations.
Leonid Andronov,Jonathan Michalon,Khalid Ouararhni,Igor Orlov,Ali Hamiche,Jean-Luc Vonesch,Bruno P. Klaholz +6 more
TL;DR: 3DClusterViSu is a method based on 3D Voronoi tessellations that allows local density estimation, segmentation and quantification of 3D SMLM data and visualization of protein clusters within a 3D tool and will favor multi-scale and multi-resolution synergies to allow integrating molecular and cellular levels in the analysis of macromolecular complexes.
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