Hyo Yang Ahn
University of Central Florida
16 Papers
81 Citations
Hyo Yang Ahn is an academic researcher from University of Central Florida. The author has contributed to research in topics: Fluorescence microscope & Chemistry. The author has an hindex of 12, co-authored 16 publications.
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Papers
High-fidelity hydrophilic probe for two-photon fluorescence lysosomal imaging.
Xuhua Wang,Dao Nguyen,Ciceron O. Yanez,Luis Rodríguez,Hyo Yang Ahn,Mykhailo V. Bondar,Kevin D. Belfield +6 more
TL;DR: LT1 has a number of properties that far exceed those of commercial lysotracker probes, including higher two-photon absorption cross sections, good fluorescence quantum yield, and, importantly, high photostability, all resulting in a superior figure of merit.
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Two-photon fluorescence microscopy imaging of cellular oxidative stress using profluorescent nitroxides
Hyo Yang Ahn,Kathryn E. Fairfull-Smith,Benjamin J. Morrow,Vanessa C. Lussini,Bosung Kim,Mykhailo V. Bondar,Steven E. Bottle,Kevin D. Belfield +7 more
TL;DR: A range of varying chromophore nitroxide free radicals and their nonradical methoxyamine analogues were synthesized and their linear photophysical properties examined in this article, where two nitroxides incorporating anthracene and fluorescein chromophores (compounds 7 and 19, respectively) exhibited two-photon absorption (2PA) cross sections of approximately 400 G.M. when excited at wavelengths greater than 800 nm.
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•Journal Article
Two-Photon Fluorescence Microscopy Imaging of Cellular Oxidative Stress Using Profluorescent Nitroxides
Hyo Yang Ahn,Kathryn E. Fairfull-Smith,Benjamin J. Morrow,Vanessa C. Lussini,Bosung Kim,Mykhailo V. Bondar,Steven E. Bottle,Kevin D. Belfield +7 more
TL;DR: The high ratio of the fluorescence quantum yield between the profluorescent nitroxide species and their nonradical adducts provides the sensitivity required for measuring a range of cellular redox environments, and their reasonable 2PA cross sections provide for the option of using two-photon fluorescence microscopy.
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Donor−Acceptor−Donor Fluorene Derivatives for Two-Photon Fluorescence Lysosomal Imaging
Sheng Yao,Hyo Yang Ahn,Xuhua Wang,Jie Fu,Eric W. Van Stryland,David J. Hagan,Kevin D. Belfield +6 more
TL;DR: Encapsulation of the new probes in Pluronic F 108NF micelles, and subsequent incubation in HCT 116 cells, resulted in very high lysosomal colocalization relative to commercial Lysotracker Red, making the micelle-encapsulated dyes particularly attractive as fluorescent probes for two-photon fluorescence microscopy lysOSomal imaging.
Near-infrared-emitting squaraine dyes with high 2PA cross-sections for multiphoton fluorescence imaging
TL;DR: New near-infrared emitting squaraine dyes exhibited a high two-photon absorption (2PA) cross-section (δ2PA), ∼20 000 GM at 800 nm, and high photostability with the photochemical decomposition quantum yield one order of magnitude lower than Cy 5, a commercially available pentamethine cyanine NIR dye.
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