Hui Xu
Tianjin University
6 Papers
34 Citations
Hui Xu is an academic researcher from Tianjin University. The author has contributed to research in topics: Gene & Chromosome. The author has an hindex of 5, co-authored 6 publications.
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Papers
In vitro DNA SCRaMbLE.
Yi Wu,Yi Wu,Rui Ying Zhu,Leslie A. Mitchell,Lu Ma,Liu Rui,Meng Zhao,Meng Zhao,Bin Jia,Hui Xu,Yun Xiang Li,Zu Ming Yang,Yuan Ma,Xia Li,Hong Liu,Duo Liu,Wen Hai Xiao,Xiao Zhou,Bing-Zhi Li,Ying-Jin Yuan,Jef D. Boeke +20 more
TL;DR: An in vitro method of DNA library construction based on structural variation is developed, enabling optimization of biosynthetic pathway flux via the rearrangement of relevant transcription units and potentially amenable to biochemical optimization in ways that the in vivo system cannot achieve.
Heterozygous diploid and interspecies SCRaMbLEing
Michael J. Shen,Yi Wu,Kun Yang,Yunxiang Li,Hui Xu,Haoran Zhang,Bing-Zhi Li,Xia Li,Wen Hai Xiao,Xiao Zhou,Leslie A. Mitchell,Joel S. Bader,Ying-Jin Yuan,Jef D. Boeke +13 more
TL;DR: A collection of heterozygous diploid strains produced by mating haploid semisynthetic Sc2.0 strains to haploid native parental strains are described and it is established that multiple sets of independent genomic rearrangements are able to lead to similar phenotype enhancements.
Optimization of ethylenediamine pretreatment and enzymatic hydrolysis to produce fermentable sugars from corn stover
Lei Qin,Xia Li,Jia-Qing Zhu,Wen-Chao Li,Hui Xu,Qi-Man Guan,Man-Tong Zhang,Bing-Zhi Li,Ying-Jin Yuan +8 more
TL;DR: In this article, a two-stage pretreatment was carried out to maximize both sugar yields and EDA removal, in which glucose and xylose enzymatic yields reached 92% and 70% respectively at 1% glucan loading.
37
Patent
Method for knocking down brewing yeast chromosome
Yuan Yingjin,Yi Wu,Sijie Zhou,Hui Xu,Yunxiang Li +4 more
- 30 Mar 2018
TL;DR: In this article, a CRISPR/Cas9 base technology was used to cut the yeast chromosome and a corresponding guide RNA was designed to guide Cas9 protein to generate an incision closeto the centromere, thus realizing the knocking down of the whole chromosome.
2
SCRaMbLEing of a Synthetic Yeast Chromosome with Clustered Essential Genes Reveals Synthetic Lethal Interactions.
Peixia Wang,Hui Xu,Hao Li,Chen Hebing,Sijie Zhou,Fangfang Tian,Bing-Zhi Li,Xiaochen Bo,Yi Wu,Ying-Jin Yuan +9 more
TL;DR: This study shows that SCRaMbLE with clustered essential genes enhances streamlining of synthetic yeast chromosome and provides a novel strategy to uncover complex genetic interactions.