Cassava ( Manihot esculenta Crantz) is a vital crop for food and economic security in many regions of the world. Despite the economic and social importance of cassava, challenges persist in developing superior varieties that meet the needs of farmers in terms of agronomic performance, nutritional quality, and resistance to pests and diseases. One of the main obstacles for genetic improvement is the lack of synchronization in flowering and the abortion of young flowers, making planned crosses and progeny production difficult. Therefore, the aim of this study was to evaluate the effect of photoperiod, premature pruning, and growth regulators on cassava flowering under low-altitude conditions in Brazil. Eight cassava clones with contrasting flowering capacity were assessed in Cruz das Almas, Bahia, using two photoperiods (ambient condition and extended photoperiod with red light for 12 hours), premature pruning at the first and second branching levels (with and without pruning), and the application of growth regulators: 0.5 mM 6-benzyladenine (BA) and 4.0 mM silver thiosulfate (STS) (with and without). Plots were assessed weekly for the number of female (NFF) and male (NMF) flowers, height of the first branching (H1B, in cm), number of days to the first branching (ND1B), and the number of branching events up to 240 days after planting (NOB). The extended photoperiod did not promote an increase in the number of flowers but allowed for precocity in cassava flowering, reducing the onset of flowering by up to 35 days, and significantly increasing the number of branches, which is closely related to flowering. The use of pruning and plant growth regulators (PGR) resulted in an increase in NFF from 2.2 (control) to 4.6 and NMF from 8.1 to 21.1 flowers. Therefore, under hot and humid tropical conditions at low altitudes in the Recôncavo of Bahia, manipulating the photoperiod and using premature pruning and plant growth regulators can accelerate cassava flowering, benefiting genetic improvement programs. 

Flowering induction in cassava using photoperiod extension premature pruning and plant growth regulators

Rapid wilting after harvest greatly decreases the ornamental and economic value of fresh-cut flowers. To determine how chlorine dioxide (ClO2) affects postharvest flower quality, Paeonia lactiflora ‘Hushui Dangxia’ cut flowers in bottles were treated with different concentrations of ClO2 (0, 25, 50, 75, and 100 mg L−1). Among the treatments, ClO2 75 (i.e., 75 mg L−1) decreased the bacterial growth and lignin content at the P. lactiflora flower stem base, while also decreasing the degree of flower stem vessel blockage. Additionally, the ClO2 75 treatment increased the relative fresh weight, water balance, soluble sugar content, soluble protein content, and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase), but decreased the malondialdehyde content and ethylene release rate of P. lactiflora cut flowers. Thus, the aging of P. lactiflora flowers was delayed, thereby maintaining the cut flower quality. Furthermore, the vase life (i.e., ornamental period) increased by three days. 

ClO2 Prolongs the Vase Life of Paeonia lactiflora ‘Hushui Dangxia’ Cut Flowers by Inhibiting Bacterial Growth at the Stem Base

This study aimed to explore the impact of L-arginine (Arg) on the development of resistance to Alternaria tenuissima (A. tenuissima) in blueberries. The metabolism of reactive oxygen species, pathogenesis-related proteins (PRs), and jasmonic acid (JA) biosynthesis pathways were analyzed, including changes in activity and gene expression of key enzymes. The results indicated that Arg treatment could prevent the development of Alternaria fruit rot in postharvest blueberries. In addition, it was also found to induce a burst of hydrogen peroxide in the blueberries early on during storage, thereby improving their resistance to A. tenuissima. Arg treatment was observed to increase the activity of antioxidant enzymes (peroxidase, catalase, superoxide dismutase, and ascorbate peroxidase) and related gene expression, as well as the total levels of phenolics, flavonoids, and anthocyanin in the blueberries. The activity and gene expression of the PRs (chitinase and β-1,3-glucanase) were elevated in Arg-treated blueberries, boosting their resistance to pathogens. Additionally, a surge in endogenous JA content was detected in Arg-treated blueberries, along with upregulated expression of key genes related the JA biosynthesis pathway (VcLOX1, VcAOS1, VcAOC, VcAOC3, VcOPR1, VcOPR3, VcMYC2, and VcCOI1), thereby further bolstering disease resistance. In conclusion, Arg treatment was determined to be a promising prospective method for controlling Alternaria fruit rot in blueberries.

Effect and Mechanism of L-Arginine against Alternaria Fruit Rot in Postharvest Blueberry Fruit

Polystichum species, native to warm-temperate and montane-tropical environments (including some alpine regions), are terrestrial or rock-dwelling ferns. These plants exhibit dark green, leather-like, and lustrous fronds, which are highly valued in floral arrangements due to their aesthetic elegance and longevity. Polystichum squarrosum is not being cultivated commercially but collected from forests for its use as cut green in the florist industry. Hence the collected material should be handled carefully to utilize every frond. Therefore, the present study aimed to develop suitable post-harvest handling procedures for this valued cut green. A Completely Randomized Design (CRD) Factorial layout with 19 treatment modules (M0 to M18) was employed. Treatment modules included packaging material as poly mesh net bags and packing materials as corrugated fiberboard boxes , two storage durations (3 and 6 days) and chemical treatments [glycerol (4%) and Benzyl adenine (25mg L-1)]. Results revealed that spraying cut fronds with 4% glycerol, wrapping them in cellophane sheets and storing them for three days (M9) resulted in significant improvements in appearance, weight change, vase life and water uptake. Fronds from Season-II (March-April 2020) exhibited excellent performance over Season-I (August-September 2019). The interaction between M9 and Season-II further enhanced the postharvest parameters. The combination of glycerol treatment and cellophane wrapping for three-day storage proved most effective in preserving the quality of cut fronds. 

Standardization of packaging material, storage durations and chemical treatments on vase life of soft shield fern Polystichum squarrosum (D. Don)

Exogenous Application of 6-Benzyladenine Increases Peanut (Arachis hypogaea L.) Pod Size by Promoting the Cell Division During the Rapid-Expansion Stage

The cytokinin 6-benzyladenine (6-BA) is widely used to regulate the growth of horticultural crops. However, it is not clear how postharvest treatment with 6-BA at various concentrations affects the quality of Chinese flowering cabbage. In this study, harvested Chinese flowering cabbage was foliar sprayed with 6-BA solution at concentrations of 5, 10, 20, 40, and 80 mg·L−1. All 6-BA treatments protected the quality of Chinese flowering cabbage during storage, and the treatment with 20 and 40 mg·L−1 6-BA showed the most obvious effect. Treatment with 6-BA reduced leaf yellowing degree and weight loss rate; maintained high chlorophyll a and chlorophyll b contents; suppressed the declines in ascorbic acid and soluble protein; enhanced antioxidant capacity; and reduced oxidative damage in cabbage leaves. Furthermore, 6-BA treatment upregulated the expression of antioxidant genes and the activities of SOD, POD, and CAT, while inhibiting the expression of senescence-related gene (BrSAG12) and chlorophyll catabolic genes (BrPAO, BrPPH, BrSGR1, BrNYC1, BrRCCR). These results suggest that postharvest 6-BA treatment enhances antioxidant capacity, delays leaf senescence, and inhibits chlorophyll degradation, thereby maintaining the quality of Chinese flowering cabbage during storage. The findings of this study provide a candidate method for preserving Chinese flowering cabbage after harvest.

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6-Benzyladenine Treatment Maintains Storage Quality of Chinese Flowering Cabbage by Inhibiting Chlorophyll Degradation and Enhancing Antioxidant Capacity

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6-Benzyladenine Treatment Maintains Storage Quality of Chinese Flowering Cabbage by Inhibiting Chlorophyll Degradation and Enhancing Antioxidant Capacity