다중혈관 관상동맥 환자에서 y-문합을 이용하여 양쪽 내흉동맥만을 사용한 우회술의 조기 성적

抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Autophagy is an important intracellular degradative process that delivers cytoplasmic proteins to lysosome for degradation. Dysfunction of autophagy is implicated in several human diseases, such as neurodegenerative diseases, infectious diseases, and cancers. Autophagy-related proteins are constitutively expressed in the eye. Increasing studies have revealed that abnormal autophagy is an important pathological feature of several ocular diseases. Pharmacological manipulation of autophagy may provide an alternative therapeutic target for some ocular diseases. In this manuscript, we reviewed the relevant progress about the role of autophagy in the pathogenesis of ocular diseases.

/pdf/repertoires-of-autophagy-in-the-pathogenesis-of-ocular-2lklvj9lx2.pdf

Repertoires of Autophagy in the Pathogenesis of Ocular Diseases

http://www.klinikaikozpont.u-szeged.hu/pedia/images/pdf/CME_TEL/Full-Text/25.pdf

The Hallmarks of Aging

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.

For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure flux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy.

Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation, it is imperative to target by gene knockout or RNA interference more than one autophagy-related protein. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways implying that not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular assays, we hope to encourage technical innovation in the field.

/pdf/guidelines-for-the-use-and-interpretation-of-assays-for-ijf2t30pbq.pdf

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Protein folding is crucial for normal physiology including development and healthy aging, and failure of this process is related to the pathology of diseases including neurodegeneration and cancer. Early thermodynamic and kinetic studies based on the unfolding and refolding equilibrium of individual proteins in the test tube have provided insight into the fundamental principles of protein folding, although the problem of predicting how any given protein will fold remains unsolved. Protein folding within cells is a more complex issue than folding of purified protein in isolation, due to the complex interactions within the cellular environment, including post-translational modifications of proteins, the presence of macromolecular crowding in cells, and variations in the cellular environment, for example in cancer versus normal cells. Development of biophysical approaches including fluorescence resonance energy transfer (FRET) and nuclear magnetic resonance (NMR) techniques and cellular manipulations including microinjection and insertion of noncanonical amino acids has allowed the study of protein folding in living cells. Furthermore, biophysical techniques such as single-molecule fluorescence spectroscopy and optical tweezers allows studies of simplified systems at the single molecular level. Combining in-cell techniques with the powerful detail that can be achieved from single-molecule studies allows the effects of different cellular components including molecular chaperones to be monitored, providing us with comprehensive understanding of the protein folding process. The application of biophysical techniques to the study of protein folding is arming us with knowledge that is fundamental to the battle against cancer and other diseases related to protein conformation or protein-protein interactions.

/pdf/studying-protein-folding-in-health-and-disease-using-148ipltam4.pdf

Studying protein folding in health and disease using biophysical approaches.

Digital fringe projection (DFP) methods are commonly used to obtain high-accuracy shape measurements. However, many measured objects may have high-contrast texture caused by edges of black- and white-colored sections of the object. In these high-contrast areas, there has consistently been a phase artifact, which in turn creates measurement error, sometimes referred to as “discontinuity-induced measurement artefacts” (DMA). Our study indicated that this error is generally shaped like a Gaussian curve. Based on this finding, we developed a method for removing this error via Gaussian curve fitting on the affected regions. These regions can be found by locating large spikes in the image intensity gradient, which directly correspond to the edge of the Gaussian artifact. We propose to use this error removal method in two ways: to remove errors on a checkerboard calibration target in order to increase calibration accuracy; and to directly remove errors in high-contrast areas to decrease shape measurement error. Experimental results demonstrate that the proposed method can successfully work for decreasing calibration error for a checkerboard calibration target, and shape measurement error can also be significantly decreased as well.

/pdf/removal-of-phase-artifacts-from-high-contrast-texture-for-3d-3a8jl2fi.pdf

Removal of phase artifacts from high-contrast texture for 3D fringe projection system

Ship wake detection can facilitate ship detection and ship motion parameters retrieval. In this paper, we propose a novel constant false alarm rate (CFAR) ship wake detection algorithm in SAR images based on length normalized scan method. As we all know, range component of ship's movement leads to azimuth offset in SAR images. The start point of wake should be located near the ship in the azimuth. According to this physical facts, length normalized scan is performed on line which may be a wake. By means of linear integral and length normalization technology, linear feature detection is transformed to point detection. Then the probability model in the scan domain is constructed to implement CFAR detection. Different resolution real SAR images are used to validate our proposed method. Experimental results show that this algorithm is effective for ship wake detection and can extract ship's velocity with good accuracy.

Ship wake CFAR detection algorithm in SAR images based on length normalized scan

Calcined Oil Shale Semi-coke for Significantly Improved Performance Alginate-Based Film by Crosslinking with Ca2+

Based on the clutter statistical characteristics of fully polarimetric SAR (PolSAR) image, this paper presents a novel change detection method using a modified heterogeneous clutter model. Firstly, a multilook product model is introduced to describe the heterogeneous clutter in PolSAR data, and a new generalized likelihood ratio test (GLRT) distance is presented to measure the degree of evolution between the statistical characteristics of multi temporal full SAR images. Compared with spherically invariant random vectors (SIRV) distance, the stochastic texture description is considered in proposed GLRT distance. Afterwards, Kittler and Illingworth (K&I) minimum error threshold segmentation method is applied to extract the changed area.

Urban change detection for high-resolution fully polarimetric SAR using a modified heterogeneous clutter model

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