Improved direct piezoelectric biosensors operating in liquid solution for the competitive label-free immunoassay of 2,4-dichlorophenoxyacetic acid

Application of bioluminescence and chemiluminescence in biomedical sciences.

New label enzymes for bioluminescent enzyme immunoassay.

Neonatal mass screening for biliary atresia: A pilot study in Tochigi Prefecture, Japan

Bioluminescent enzyme immunoassay using thermostable mutant luciferase and acetate kinase as a labelled enzyme

A sensitive bioluminescent enzyme immunoassay (BLEIA) has been developed using acetate kinase(AK) as a new label enzyme. The bioluminescent assay of AK was done by firefly luciferase for measuring ATP produced by enzymatic reaction of AK using acetylphosphate and ADP as substrate. The detection limit of AK was 60 zmol/assay(1.2 amol/ml) and the coefficient of variation (CV, n=5) for each point, ranged from 2.1 to 8.3%. The proposed bioluminescent assay of AK was very simple, rapid and highly sensitive. The AK assay developed here, was applied to BLEIA of 17 α-hydroxyprogesterone, rabbit IgG and human thyroid stimulating hormone(TSH) as model compound for competitive and immunometric immunoassay.

Development of New Label Enzyme for Bioluminescent Enzyme Immunoassay

ELISA (Enzyme-Linked Immunosorbent Assay) for Glycocholic Acid in Neonatal Blood Spotted on Filter Paper

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Development of New Label Enzyme for Bioluminescent Enzyme Immunoassay

ELISA (Enzyme-Linked Immunosorbent Assay) for Glycocholic Acid in Neonatal Blood Spotted on Filter Paper